Detailed information of TA system TA206971

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2261088..2261724
• Replicon: chromosome
• Accession: NZ_CP077291
• Organism: Citrobacter farmeri strain FDAARGOS 1423

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A2K9P8U1
• Locus tag: I6L53_RS10655
• Protein ID: WP_072015688.1
• Coordinates: 2261088..2261279 (+)
• Length: 64 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: I6L53_RS10660
• Protein ID: WP_125368572.1
• Coordinates: 2261308..2261724 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6L53_RS10635 (I6L53_10635)	2256279..2258240	+	1962	WP_042318936.1	U32 family peptidase	-
I6L53_RS10640 (I6L53_10640)	2258281..2258409	-	129	WP_071887674.1	DUF2474 domain-containing protein	-
I6L53_RS10645 (I6L53_10645)	2258409..2259419	-	1011	WP_042318937.1	cytochrome d ubiquinol oxidase subunit II	-
I6L53_RS10650 (I6L53_10650)	2259419..2260822	-	1404	WP_042318938.1	cytochrome ubiquinol oxidase subunit I	-
I6L53_RS10655 (I6L53_10655)	2261088..2261279	+	192	WP_072015688.1	type II toxin-antitoxin system HicA family toxin	Toxin
I6L53_RS10660 (I6L53_10660)	2261308..2261724	+	417	WP_125368572.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
I6L53_RS10665 (I6L53_10665)	2261812..2263221	+	1410	WP_042318940.1	PLP-dependent aminotransferase family protein	-
I6L53_RS10670 (I6L53_10670)	2263546..2264691	+	1146	WP_042318941.1	ABC transporter substrate-binding protein	-
I6L53_RS10675 (I6L53_10675)	2264709..2265722	+	1014	WP_042318942.1	ABC transporter ATP-binding protein	-
I6L53_RS10680 (I6L53_10680)	2265723..2266667	+	945	WP_042318943.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-65)

Sequences

Toxin

Protein

• Length: 64 a.a.
• Molecular weight: 7177.36 Da
• Isoelectric Point: 11.6208

>T206971 WP_072015688.1 NZ_CP077291:2261088-2261279 [Citrobacter farmeri]
VKQSEFRRWLESQGVGVTNGSHHLKLRYRGKRSVMPRHPGDEIKEALRKAILKQLGLSPSSLI

Nucleotide

• Length: 192 bp

>T206971 NZ_CP102832:16390-16677 [Acinetobacter baumannii]
ATGGAACAGTATTTTGAATGGGATGAGGCTAAGAATCGAAAGAATCAGAAAAAGCATGATATCTCTTTCGAAACTGCAAG
CCTTGTTTTTGAAGATCCATTACGGATCTCAATCCAAGACAGATATACTAATGGTGAAGAACGTTGGCAAACCATTGGAC
GAGTTAAAGGCGTACTAATGTTATTGGTGGCTCACACCATTTTTGATGAAGATGACTGTGAAATCATCCGAATCATTAGT
GCAAGGCAAGTCACTAAAGCGGAGCGAAACAAATATGAGCATGGTTAG

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15219.47 Da
• Isoelectric Point: 4.9310

>AT206971 WP_125368572.1 NZ_CP077291:2261308-2261724 [Citrobacter farmeri]
MRYPVHLVPAPEGGYVVSFPDIPEALTQGETRQQAIDTALEALITAFEFYFEDNQSVPLPTPVKEGDDFVEIPLSVASKV
LLLNAFLASKISQQELAKRIGRPKQEITRLFDLRHATKIDAVQTAARALGKELSLTLS

Nucleotide

• Length: 417 bp

>AT206971 NZ_CP102832:16664-16978 [Acinetobacter baumannii]
ATGAGCATGGTTAGATACTCACACAAAGAGCTGAATGAGAAATTTGGTGAGAAGCAAGATGCTGAAATTCAACGCTTGCT
TGCTAAAGGCACGGTTCCTGATGATCAGCTAGATCTATCAGATATTCCTGAAATTACAGACTGGAGCAATGCTGTACGCC
AAAATCAATTTTACCGCCCAGTAAAGCAACAAACTTCAATTCGGTTAGATGCTGATGTACTTGCTTGGTTCAAAGCACAA
GGCAAAGGTTATCAAACACGTATGAATGAAATTTTACGAGATGCTATGCTAAAAGAATTAAAAAATCATCAATAA

Structures

Toxin

• AlphaFold DB: A0A2K9P8U1