Detailed information of TA system TA206962

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 1781077..1781667
• Replicon: chromosome
• Accession: NZ_CP077291
• Organism: Citrobacter farmeri strain FDAARGOS 1423

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: I6L53_RS08340
• Protein ID: WP_042318227.1
• Coordinates: 1781077..1781409 (-)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: A0A8H9TUM3
• Locus tag: I6L53_RS08345
• Protein ID: WP_042318230.1
• Coordinates: 1781410..1781667 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6L53_RS08310 (I6L53_08310)	1776150..1776893	+	744	WP_042318223.1	adenosylcobinamide-GDP ribazoletransferase	-
I6L53_RS08315 (I6L53_08315)	1776919..1777986	+	1068	WP_042318224.1	nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase	-
I6L53_RS08320 (I6L53_08320)	1778116..1779048	+	933	WP_094465565.1	L,D-transpeptidase	-
I6L53_RS08330 (I6L53_08330)	1779346..1780833	+	1488	WP_042318226.1	EmmdR/YeeO family multidrug/toxin efflux MATE transporter	-
I6L53_RS08340 (I6L53_08340)	1781077..1781409	-	333	WP_042318227.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
I6L53_RS08345 (I6L53_08345)	1781410..1781667	-	258	WP_042318230.1	antitoxin	Antitoxin
I6L53_RS23605	1782279..1784216	+	1938	WP_232231063.1	hypothetical protein	-
I6L53_RS23610	1784120..1786177	+	2058	WP_232231064.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-108)

Antitoxin

• Antitoxin PemI-like (4-84)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11741.57 Da
• Isoelectric Point: 8.0290

>T206962 WP_042318227.1 NZ_CP077291:c1781409-1781077 [Citrobacter farmeri]
MDRGEIWLVSLDPTAGHEQSGKRPVLIVSLASFNMLTQLPVVVPVTSGGNFARSAGFTVSLDGAGTKTTGVIRCDQPRTI
DMGARNGKRLERIPDAVVNEVLARLEAILI

Nucleotide

• Length: 333 bp

>T206962 NZ_CP102827:c2713968-2713865 [Salmonella enterica subsp. enterica serovar Indiana]
GGCAAGGCGATTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9369.87 Da
• Isoelectric Point: 4.5821

>AT206962 WP_042318230.1 NZ_CP077291:c1781667-1781410 [Citrobacter farmeri]
MYTTRLKKVGGSVMLAVPPAVLKTLALSTDSEVGMTIDNGCLIIEPQKRPRYSLEELLAQCDPHAEMSEEDREWINAPAV
GKEIL

Nucleotide

• Length: 258 bp

>AT206962 NZ_CP102827:2713863-2714008 [Salmonella enterica subsp. enterica serovar Indiana]
ATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGC
ATTAATGCAGGCTAAATCGCCTTGCCCTTTAAGAATAGATGACGACGCCAGGTTTTCCAGTTTGCG