Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 726307..726883 Replicon chromosome
Accession NZ_CP077291
Organism Citrobacter farmeri strain FDAARGOS 1423

Toxin (Protein)


Gene name shpA Uniprot ID -
Locus tag I6L53_RS03375 Protein ID WP_042321950.1
Coordinates 726307..726594 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A8H9NUP2
Locus tag I6L53_RS03380 Protein ID WP_042321954.1
Coordinates 726581..726883 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6L53_RS03360 (I6L53_03360) 722215..722937 - 723 WP_084196626.1 restriction endonuclease subunit S -
I6L53_RS03365 (I6L53_03365) 723075..723611 - 537 WP_052425403.1 restriction endonuclease subunit S -
I6L53_RS03370 (I6L53_03370) 723611..725953 - 2343 WP_042321949.1 class I SAM-dependent DNA methyltransferase -
I6L53_RS03375 (I6L53_03375) 726307..726594 + 288 WP_042321950.1 BrnT family toxin Toxin
I6L53_RS03380 (I6L53_03380) 726581..726883 + 303 WP_042321954.1 BrnA antitoxin family protein Antitoxin
I6L53_RS03385 (I6L53_03385) 726953..727912 - 960 WP_042321957.1 GTPase -
I6L53_RS03390 (I6L53_03390) 727925..728128 - 204 WP_024130742.1 YbdD/YjiX family protein -
I6L53_RS03395 (I6L53_03395) 728180..730330 - 2151 WP_042321961.1 pyruvate/proton symporter BtsT -
I6L53_RS03400 (I6L53_03400) 730627..731634 + 1008 WP_042321963.1 Ldh family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(43-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11111.57 Da        Isoelectric Point: 8.6421

>T206960 WP_042321950.1 NZ_CP077291:726307-726594 [Citrobacter farmeri]
MPTEFEWDANKAKSNVGKHGVRFEDAILVFDDPRHLSRQDRIENGEYRWQTLGLVHSIVVILVAHSIRFESGIEVIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T206960 NZ_CP102827:c1351942-1351724 [Salmonella enterica subsp. enterica serovar Indiana]
ATGTCTGATAAACCATTAACTAAAACTGATTATTTGATGCGTTTACGGCGCTGTCAGACAATTGACACTCTGGAGCGCGT
CATTGAGAAAAATAAATATGAATTGTCTGACAATGAGCTGGCTGTATTTTACTCAGCTGCGGATCACCGTCTTGCAGAAT
TGACGATGAACAAACTCTACGACAAGATCCCCTCTTCAGTATGGAAATTCATTCGTTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11562.18 Da        Isoelectric Point: 9.9265

>AT206960 WP_042321954.1 NZ_CP077291:726581-726883 [Citrobacter farmeri]
MSMVKHKRGTQSALSAQHEVELKALAKQSDDEIDYSDIPFSPDEQWSEAVRGKFFRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLREQNKK

Download         Length: 303 bp

>AT206960 NZ_CP102827:c1352344-1351970 [Salmonella enterica subsp. enterica serovar Indiana]
ATGGACGAATACTCACCCAAAAGACATGATATAGCACAGCTTAAATTTCTTTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAACCATGGCTGGGTTAATGACCCGACCTCAGCAGTCAACCTGCAGCTCAATGAGCTGATAGAGC
ATATTGCAACTTTCGCGCTTAATTATAAAATTAAGTACAATGAAGACAATAAATTGATTGCGCAGATAGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTATGGTATTAATACGCAGGATTTGCAAAAATGGCGTAAATCGGGCAACCGCCT
GTTCCGTTGTTTCGTCAACGCCACCAGGGCCAATCCTGTTAGTTTGTCTTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References