Detailed information of TA system TA206941

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 142329..143065
• Replicon: plasmid unnamed
• Accession: NZ_CP077280
• Organism: Klebsiella quasipneumoniae strain FDAARGOS 1443

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A142I508
• Locus tag: I6L73_RS25940
• Protein ID: WP_032720647.1
• Coordinates: 142583..143065 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: I6L73_RS25935
• Protein ID: WP_063106370.1
• Coordinates: 142329..142595 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6L73_RS25920 (I6L73_25920)	138732..139898	+	1167	WP_136084789.1	hypothetical protein	-
I6L73_RS25925 (I6L73_25925)	140618..141900	+	1283	Protein_148	IS3 family transposase	-
I6L73_RS25930 (I6L73_25930)	141885..142022	+	138	WP_226648960.1	cell envelope integrity TolA C-terminal domain-containing protein	-
I6L73_RS25935 (I6L73_25935)	142329..142595	+	267	WP_063106370.1	DUF1778 domain-containing protein	Antitoxin
I6L73_RS25940 (I6L73_25940)	142583..143065	+	483	WP_032720647.1	GNAT family N-acetyltransferase	Toxin
I6L73_RS25945 (I6L73_25945)	143281..144681	+	1401	WP_063106369.1	ISNCY family transposase	-
I6L73_RS25950 (I6L73_25950)	144869..145288	+	420	WP_012540023.1	ASCH domain-containing protein	-
I6L73_RS25955 (I6L73_25955)	145870..146871	+	1002	WP_040107896.1	LacI family DNA-binding transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..151573	151573
	-	inside	IScluster/Tn	-	-	137899..144681	6782

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17335.02 Da
• Isoelectric Point: 9.8720

>T206941 WP_032720647.1 NZ_CP077280:142583-143065 [Klebsiella quasipneumoniae]
VGRITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKKGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T206941 NZ_CP102780:1403591-1404499 [Pandoraea commovens]
ATGCAAGCCGAAGCCCATCAACACGAAGTGCCAGAAGACGATGTCGTCGCGCCCTTGCGCGCACGGCTGGACGCGTTTCC
GTATCGCACAGTCGCCACGCGGGCGGGCACCGTCGGTTATCGCTGCGTGGGCACTGAGAACGACGCCGGTGTGCCGGTCG
TGATGCTGCACGGCATCGGCTCCGGCGCGGCGTCCTGGTTGGCGCAGTTCGAAGCCACGGGGCTCGATGCAAAGCTGTAC
GCCTGGGATGCACCCGGCTACGGCGCGACGTCGAATGTCGCTGCCGCCGAACCGCATCCCGACGCCTATGCCGACGCCCT
TGAAGCGTGGCTCGACACGCTCGGTCTGGATCGTGTGGCGCTGGTGGGCCATTCGCTGGGCGCGATCATGGCAACGAAGT
TTGCAAGCCGCGCGCCGCAACGCGTGCGCGGCCTCTTTCTCTGCTCACCAGCCAATGGCTACGCCCGCGCCGATGCTGAG
GTACGTCTGTCCAAGCGCGACAGCCGTCTGGCGATGCTCGACAAGCTCGGTCCCGCAGGCATGGCGCGTGAGCGAAGTGA
CAACCTCGTCGCGCCCGGCGCCGCCGATCTGCCGCGCGCGTGGGTGAAATGGAATATGTCGCGGGTGTTGCCGGTCGGCT
ACCGTCAGGCGACGCACCTGCTCTCGAATGGCGACGTCGCTGGCGAACTCGCACAGTACGTGAGCGCCAAGGCAGGTCCC
GTTGCGGTGGCCGTGGGTGCGCAGGACGGCATCACGTCGCCTGCTGCTTGCGAGGTCATCGCGAAGGTCGCGGGCGTGCC
GCTGCAAATCATCGACGGTGCAGGCCACGCGTCGTACATCGAAACGCCGGACGTTCTGAATGCTGCGCTCGCCGCGTGGC
TCGAACGTGTCGCGGCAGGGGGGCGTTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10151.58 Da
• Isoelectric Point: 4.5256

>AT206941 WP_063106370.1 NZ_CP077280:142329-142595 [Klebsiella quasipneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDETAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT206941 NZ_CP102780:1404549-1405427 [Pandoraea commovens]
ATGCATGACGATCAAAACGTCTCGCTCGATGGCGATGCCGTCGTCGAAGGCGCCGCCGAGTCCCGCTACCTCGTGCCGGG
TCTTGAGCGCGGCCTGCGCATCCTCACGCAATTCTCGCCGCGCGAACCGGTGTTCAGTGCACCGGAACTGTCGCAACGGC
TGGGCATTCCGCGTACGACGGTCTTCCGTCTGTTGCAGACGCTCGAAGCCATGGGCTTTCTTGAGCGCGCAGGAAGCGAA
AAGAACTTCCGTCTGGGCGTGGCCGTGCTGCGTCTGGGCTTCGAATACCTCAGCTCGCTGGAACTGACCGACCTCGGCAT
TCCGATCATCGAGCGCCTGCGCGACGCGACCCAACTGACGTCGCACATCGTGATTCGCGACGGCCGCGACATCGTGTTCG
TCGCGAAGGCGCAAAGCGTGGCGGGCGTCTTCAACTCGATCAAGGTCAACGTCGGCACGCGTTTGCCTGCGCACGCCACG
GTGCACGGTCACGTGCTGATGGGCGATCTGACGCTCACCGAACTGCGTGCGCTCTATCCCGAGAAGACGCTGGAGAAATT
CACGCCGCAGACGCCCGAGTCGCTGACCGAACTGTTCGAGCGCGTGCGCGAAGACGCCGAGCGCGGCTATGCCGTGTCGG
CCTCGTCGTTCGAGCGCGGCATCAGCGTGGTGAGCGCACCGGTGCGCAACGACGTGGGCCGCATCTGCGCGGCCATCACG
GTCACGATTCCGCGCGCCAATATCGACGCATCGATGCTCGACGCGGGCCTGATCGGTCAGGTGCGCGAAGCGGCCGATGC
GCTCTCGCACCGTCTGAATTACCGCCCGGGCAGCGACGGCAAGTCCACCCCCTACATGAAGTCACTGGGACTCAAATGA

Structures

Toxin

• AlphaFold DB: A0A142I508