Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/ElaA-DUF1778
Location 85666..86405 Replicon plasmid unnamed
Accession NZ_CP077280
Organism Klebsiella quasipneumoniae strain FDAARGOS 1443

Toxin (Protein)


Gene name tacT Uniprot ID A0A5E1AVR5
Locus tag I6L73_RS25655 Protein ID WP_013087172.1
Coordinates 85666..86151 (-) Length 162 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID A0A5E1AWX8
Locus tag I6L73_RS25660 Protein ID WP_012540086.1
Coordinates 86139..86405 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6L73_RS25605 (I6L73_25605) 81047..81460 - 414 WP_136084777.1 helix-turn-helix domain-containing protein -
I6L73_RS25610 (I6L73_25610) 81461..81739 - 279 WP_004152721.1 helix-turn-helix transcriptional regulator -
I6L73_RS25615 (I6L73_25615) 81729..82049 - 321 WP_004152720.1 type II toxin-antitoxin system RelE/ParE family toxin -
I6L73_RS25620 (I6L73_25620) 82130..82354 - 225 WP_063106387.1 hypothetical protein -
I6L73_RS25625 (I6L73_25625) 82365..82577 - 213 WP_063106386.1 hypothetical protein -
I6L73_RS25630 (I6L73_25630) 82638..82967 - 330 WP_162544171.1 hypothetical protein -
I6L73_RS26145 82954..83199 - 246 WP_074155869.1 hypothetical protein -
I6L73_RS25635 (I6L73_25635) 83838..84155 - 318 WP_038807150.1 hypothetical protein -
I6L73_RS25640 (I6L73_25640) 84170..84520 - 351 WP_063106385.1 hypothetical protein -
I6L73_RS25645 (I6L73_25645) 84517..84786 - 270 WP_038807148.1 hypothetical protein -
I6L73_RS25650 (I6L73_25650) 85164..85283 - 120 WP_223230170.1 Hok/Gef family protein -
I6L73_RS26150 85231..85383 - 153 Protein_90 DUF5431 family protein -
I6L73_RS25655 (I6L73_25655) 85666..86151 - 486 WP_013087172.1 GNAT family N-acetyltransferase Toxin
I6L73_RS25660 (I6L73_25660) 86139..86405 - 267 WP_012540086.1 DUF1778 domain-containing protein Antitoxin
I6L73_RS25665 (I6L73_25665) 86705..87028 + 324 WP_012540250.1 metalloregulator ArsR/SmtB family transcription factor -
I6L73_RS25670 (I6L73_25670) 87030..87743 + 714 WP_012540184.1 arsenical resistance protein ArsH -
I6L73_RS25675 (I6L73_25675) 87752..88297 + 546 WP_012540227.1 sigma-70 family RNA polymerase sigma factor -
I6L73_RS25680 (I6L73_25680) 88373..88735 + 363 WP_012540155.1 arsenic metallochaperone ArsD family protein -
I6L73_RS25685 (I6L73_25685) 88762..90513 + 1752 WP_162866166.1 arsenical pump-driving ATPase -
I6L73_RS25690 (I6L73_25690) 90638..91174 + 537 WP_012540238.1 GNAT family N-acetyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..151573 151573


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(27-152)

Antitoxin

Vibrio phage ICP1, Orf50

(2-87)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17810.62 Da        Isoelectric Point: 9.8560

>T206940 WP_013087172.1 NZ_CP077280:c86151-85666 [Klebsiella quasipneumoniae]
VGRVTVPEPLSSFHQVAEFVCGETVLDDWLKQKGLKNQALGSARTFVVCKKDTKQVAGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDLSFRGKGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKNFYIHHGFKPSQTQQRTLFLKLP
Q

Download         Length: 486 bp

>T206940 NZ_CP102780:186353-186772 [Pandoraea commovens]
ATGTACCTACTCGATACCAACGTCGTCAGCGAGAGTCGTAAGCGCGGTGGTGGCGACGAGGGTGTTCAGCGTTTCATGCG
AATGGCAGAACGGCGTCGAGCGCAGAAATTCATTTCGGTGATCACGATGGGTGAGTTGCAGCGCGGCATGAGAGTGCTGC
GGCACAGAAACGATGTTCGACAAGCGTCGCTTATCGAATCATGGTTGGAGCAGCTTCACATTGAATATGCCGACGACATT
TTGCCGATAGATCTCACGATATGTAACGCTTGGGCGCGCATGCGCGTGCCCAACGAGCAACATCCGATAGACAAACTCAT
TGCCGCGACTGCGCTGGTGCACGAACTCATCGTCGTCACACGCAACGTCAAAGATTTTTCCGACACCGGAGTGGAGGTAT
ACAACCCGTTTCAGCCGTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10076.47 Da        Isoelectric Point: 4.3721

>AT206940 WP_012540086.1 NZ_CP077280:c86405-86139 [Klebsiella quasipneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIDMLDAPVADDPAIDKLL
ARKPQWDV

Download         Length: 267 bp

>AT206940 NZ_CP102780:186138-186338 [Pandoraea commovens]
ATGATGTTGATTGAAGGGATCGACGAGCAGTTGATGCGGTCGATTGAGTCGCGGGCTGCGCAGGGCGGAAGGACGCCGGA
AGAGGAAGTGCTGCAGATTCTTGATCGAGTGGCGCGGGTTCCGCGCTTCCGATCGCTGGGAGAAGCACTGCGAGCCATGC
CGAACGTCGGTCTGGATAGCGACTTCGAGCGGATTAACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5E1AVR5

Antitoxin

Source ID Structure
AlphaFold DB A0A5E1AWX8

References