Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemK-mazE/MazF(toxin)
Location 920292..920921 Replicon chromosome
Accession NC_003212
Organism Listeria innocua Clip11262

Toxin (Protein)


Gene name pemK Uniprot ID Q92DC7
Locus tag LIN_RS04535 Protein ID WP_010990666.1
Coordinates 920574..920921 (+) Length 116 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID H1GC21
Locus tag LIN_RS04530 Protein ID WP_003761302.1
Coordinates 920292..920570 (+) Length 93 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LIN_RS04510 915589..917073 + 1485 WP_010990663.1 PH domain-containing protein -
LIN_RS04515 917224..918603 + 1380 WP_010990664.1 protoporphyrinogen oxidase -
LIN_RS04520 918605..918961 + 357 WP_003761296.1 holo-ACP synthase -
LIN_RS04525 918980..920086 + 1107 WP_010990665.1 alanine racemase -
LIN_RS04530 920292..920570 + 279 WP_003761302.1 CopG family ribbon-helix-helix protein Antitoxin
LIN_RS04535 920574..920921 + 348 WP_010990666.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
LIN_RS04540 921173..922009 + 837 WP_003771126.1 STAS domain-containing protein -
LIN_RS04545 922015..922371 + 357 WP_003721457.1 STAS domain-containing protein -
LIN_RS04550 922374..922784 + 411 WP_010990667.1 anti-sigma regulatory factor -
LIN_RS04555 922801..923805 + 1005 WP_010990668.1 PP2C family protein-serine/threonine phosphatase -
LIN_RS04560 923955..924299 + 345 WP_003721460.1 anti sigma b factor antagonist RsbV -
LIN_RS04565 924283..924756 + 474 WP_003761314.1 anti-sigma B factor RsbW -
LIN_RS04570 924734..925513 + 780 WP_010990669.1 RNA polymerase sigma factor SigB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-109)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 12801.85 Da        Isoelectric Point: 6.4735

>T20694 WP_010990666.1 NC_003212:920574-920921 [Listeria innocua Clip11262]
MMVKRGDVYYADLSPVVGSEQGGIRPVLIIQNDIGNRFSPTVIVAAITAKIQKAKLPTHVEATRKDGFERDSVILLEQIR
TIDKQRLTDKITHLDEELMAKVNQALEVSLGVVEF

Download         Length: 348 bp

>T20694 NC_003212:920574-920921 [Listeria innocua Clip11262]
CTGATGGTGAAGCGTGGTGACGTTTACTACGCGGACCTTTCCCCCGTGGTCGGAAGCGAGCAAGGGGGAATAAGGCCTGT
TCTCATCATTCAAAATGATATTGGTAATAGATTCAGTCCAACTGTGATCGTGGCAGCAATAACTGCAAAAATTCAAAAAG
CGAAGTTGCCAACACATGTGGAAGCTACTCGTAAAGACGGCTTTGAAAGAGATTCTGTCATTCTATTAGAACAAATTAGA
ACGATAGATAAACAACGCCTGACAGATAAGATTACACATTTGGACGAGGAACTAATGGCCAAGGTAAATCAGGCACTTGA
AGTCAGTCTAGGAGTAGTAGAATTCTAA

Antitoxin


Download         Length: 93 a.a.        Molecular weight: 10605.13 Da        Isoelectric Point: 4.2980

>AT20694 WP_003761302.1 NC_003212:920292-920570 [Listeria innocua Clip11262]
VLEKEKRMIISVELTQEMVQELDVVVEKEKMGRSEVIMEATQQFLQEKRARELRDEMERGYAEMATINFAIACECTHVEA
EAEDRNISILGG

Download         Length: 279 bp

>AT20694 NC_003212:920292-920570 [Listeria innocua Clip11262]
GTGTTAGAGAAAGAAAAACGGATGATAATATCCGTAGAACTGACACAGGAAATGGTACAAGAACTCGACGTAGTTGTAGA
AAAAGAAAAAATGGGGCGGAGTGAAGTTATAATGGAAGCAACGCAACAATTTTTACAGGAGAAAAGAGCTCGTGAATTAA
GAGACGAGATGGAACGCGGCTATGCAGAAATGGCGACGATTAATTTCGCTATCGCGTGCGAGTGTACTCATGTCGAAGCA
GAGGCAGAAGACAGGAATATTAGTATTTTAGGAGGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1E7E4N0

Antitoxin

Source ID Structure
AlphaFold DB H1GC21

References