Detailed information of TA system TA206905

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 3376515..3377131
• Replicon: chromosome
• Accession: NZ_CP077264
• Organism: Enterobacter hormaechei strain FDAARGOS 1434

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: I6L64_RS16055
• Protein ID: WP_077266491.1
• Coordinates: 3376760..3377131 (+)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A6G4MTK3
• Locus tag: I6L64_RS16050
• Protein ID: WP_015569912.1
• Coordinates: 3376515..3376757 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6L64_RS16020 (I6L64_16020)	3371574..3372374	+	801	WP_003861944.1	DeoR/GlpR family DNA-binding transcription regulator	-
I6L64_RS16025 (I6L64_16025)	3372493..3373092	+	600	WP_003861947.1	glucose-1-phosphatase	-
I6L64_RS16030 (I6L64_16030)	3373086..3373967	+	882	WP_003861949.1	virulence factor BrkB family protein	-
I6L64_RS16035 (I6L64_16035)	3373964..3374401	+	438	WP_015569909.1	D-aminoacyl-tRNA deacylase	-
I6L64_RS16040 (I6L64_16040)	3374446..3375387	+	942	WP_015569910.1	fatty acid biosynthesis protein FabY	-
I6L64_RS16045 (I6L64_16045)	3375396..3376316	-	921	WP_058687689.1	alpha/beta hydrolase	-
I6L64_RS16050 (I6L64_16050)	3376515..3376757	+	243	WP_015569912.1	ribbon-helix-helix domain-containing protein	Antitoxin
I6L64_RS16055 (I6L64_16055)	3376760..3377131	+	372	WP_077266491.1	type II toxin-antitoxin system VapC family toxin	Toxin
I6L64_RS16060 (I6L64_16060)	3377171..3378100	-	930	WP_006808712.1	formate dehydrogenase accessory protein FdhE	-
I6L64_RS16065 (I6L64_16065)	3378097..3378732	-	636	WP_015569914.1	formate dehydrogenase cytochrome b556 subunit	-
I6L64_RS16070 (I6L64_16070)	3378729..3379631	-	903	WP_014072386.1	formate dehydrogenase subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-115)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-49)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13723.85 Da
• Isoelectric Point: 6.4882

>T206905 WP_077266491.1 NZ_CP077264:3376760-3377131 [Enterobacter hormaechei]
MEHMAVFDTNILIDLFNNRVEAADAIEHTASHRAISLITWMEVMVGARRHGHEAKTAAVMGAFEIIDVSRDIAERSVLLR
EKHGMKLPDAIILATAQSRKCPLISRNTKDFAGIDEVLTPYQV

Nucleotide

• Length: 372 bp

>T206905 NZ_CP102749:3855389-3855694 [Pectobacterium parvum]
ATGCAATTCATTGTTTATGAATATAAAAGAACCAGTAACTACAAGATGTTTGTTGATGTACAAAGCGATATTGTTGAAAC
ACCCAAGCGGCGCATGGTCATTCCGCTTACCGAAGCACATCACCTGTCTGAGAAAGTGAATAAAATGCTGTTTCCTCTGA
TCCGCATCGATGCGGAAGATTATCGGCTGATGACAACTGAACTATCGAGCGTTCCTGTTGAGGTGATTGGTGAGGCTATT
GCGGATCTTGGGGAATATGCAGATGAGATTAAGGATGCCATTAATCTTATGTTTTGGGGGATTTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9440.72 Da
• Isoelectric Point: 4.4076

>AT206905 WP_015569912.1 NZ_CP077264:3376515-3376757 [Enterobacter hormaechei]
MSVLAELDMGRILIDLSDDVIQRLDNLKQLRNQPRAELLREAIELYLDQQSSSVIRNALGLWGDRKEDGLEYERKLREEW

Nucleotide

• Length: 243 bp

>AT206905 NZ_CP102749:3855168-3855386 [Pectobacterium parvum]
ATGAAACACCGTGTCAGCGTTACCGTGGACAAAGACAATTATCAGGTCCTGAGTGCCGCCGGAGTCAATATTTCCGGGCT
GGTGAATGACGCCATTGGCAAAGAAGCCCGCCGCATCAAGGCTGAGGAGTGGAAGAAGGAAAACCGAGAAGGGATGGAAG
AAGTCGCCCGATTTATCGCGCAGAATGGCTCCTTTGCAGATGAAAACAGGAATTGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A6G4MTK3