Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 122215..122831 Replicon chromosome
Accession NZ_CP077262
Organism Citrobacter pasteurii strain FDAARGOS 1424

Toxin (Protein)


Gene name vapC Uniprot ID A0A6N6JZM7
Locus tag I6L54_RS00630 Protein ID WP_005121821.1
Coordinates 122215..122589 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A923DW40
Locus tag I6L54_RS00635 Protein ID WP_005121822.1
Coordinates 122589..122831 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6L54_RS00615 (I6L54_00615) 119718..120620 + 903 WP_005121817.1 formate dehydrogenase O subunit beta -
I6L54_RS00620 (I6L54_00620) 120617..121252 + 636 WP_040233076.1 formate dehydrogenase cytochrome b556 subunit -
I6L54_RS00625 (I6L54_00625) 121249..122178 + 930 WP_005121819.1 formate dehydrogenase accessory protein FdhE -
I6L54_RS00630 (I6L54_00630) 122215..122589 - 375 WP_005121821.1 type II toxin-antitoxin system VapC family toxin Toxin
I6L54_RS00635 (I6L54_00635) 122589..122831 - 243 WP_005121822.1 CopG family transcriptional regulator Antitoxin
I6L54_RS00640 (I6L54_00640) 123037..123966 + 930 WP_005121823.1 alpha/beta hydrolase -
I6L54_RS00645 (I6L54_00645) 124052..124363 + 312 WP_040233077.1 type II toxin-antitoxin system HigB family toxin -
I6L54_RS00650 (I6L54_00650) 124360..124806 + 447 WP_040233078.1 helix-turn-helix domain-containing protein -
I6L54_RS00655 (I6L54_00655) 124818..125759 - 942 WP_003825286.1 fatty acid biosynthesis protein FabY -
I6L54_RS00660 (I6L54_00660) 125804..126241 - 438 WP_003028671.1 D-aminoacyl-tRNA deacylase -
I6L54_RS00665 (I6L54_00665) 126238..127110 - 873 WP_040233081.1 virulence factor BrkB family protein -
I6L54_RS00670 (I6L54_00670) 127104..127703 - 600 WP_040233083.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Ribbon-helix-helix protein, CopG

(13-47)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13655.81 Da        Isoelectric Point: 8.0276

>T206876 WP_005121821.1 NZ_CP077262:c122589-122215 [Citrobacter pasteurii]
MVKGSALFDTNILIDLFSGRSEAKQAIETWPPQNAISLITWMEVMVGAKKYHQESCTRVALSAFNVIGVSQDIAERSVSI
RQEYGMKLPDAIILATAQIHRLTLVTRNTKDFAGLSGVVTPYTL

Download         Length: 375 bp

>T206876 NZ_CP102740:31564-31716 [Salmonella enterica subsp. enterica serovar Kentucky]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCATCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGGACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9396.71 Da        Isoelectric Point: 4.3543

>AT206876 WP_005121822.1 NZ_CP077262:c122831-122589 [Citrobacter pasteurii]
MMAGMDMGRILLDLSDEVIKRLDDLKVQRNLPRAELLREAVEQYLERQEQAETTISSALGLWKGCEEDGVEYQRKLREEW

Download         Length: 243 bp

>AT206876 NZ_CP102740:c31509-31452 [Salmonella enterica subsp. enterica serovar Kentucky]
GCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6N6JZM7

Antitoxin

Source ID Structure

References