Detailed information of TA system TA206851

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 2375940..2376516
• Replicon: chromosome
• Accession: NZ_CP077242
• Organism: Escherichia fergusonii strain FDAARGOS 1438

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: I6L68_RS11280
• Protein ID: WP_024256621.1
• Coordinates: 2375940..2376227 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: A0A829G8Z0
• Locus tag: I6L68_RS11285
• Protein ID: WP_000063156.1
• Coordinates: 2376214..2376516 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6L68_RS11270 (2371275)	2371275..2374787	-	3513	WP_000981390.1	type I restriction-modification system endonuclease	-
I6L68_RS11275 (2375050)	2375050..2375784	+	735	WP_000208600.1	hypothetical protein	-
I6L68_RS11280 (2375940)	2375940..2376227	+	288	WP_024256621.1	BrnT family toxin	Toxin
I6L68_RS11285 (2376214)	2376214..2376516	+	303	WP_000063156.1	BrnA antitoxin family protein	Antitoxin
I6L68_RS11290 (2376550)	2376550..2377506	-	957	WP_001297640.1	GTPase	-
I6L68_RS11295 (2377517)	2377517..2377720	-	204	WP_000467859.1	YbdD/YjiX family protein	-
I6L68_RS11300 (2377770)	2377770..2379920	-	2151	WP_001314407.1	pyruvate/proton symporter BtsT	-
I6L68_RS11305 (2380190)	2380190..2380702	-	513	WP_001175454.1	4-hydroxyphenylacetate 3-monooxygenase reductase subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (37-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11267.72 Da
• Isoelectric Point: 6.8816

>T206851 WP_024256621.1 NZ_CP077242:2375940-2376227 [Escherichia fergusonii]
MPMEFEWDANKAKSNRVKHDIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Nucleotide

• Length: 288 bp

>T206851 NZ_CP102719:225994-226305 [Salmonella enterica subsp. enterica serovar Kentucky]
ATGCAATTTATAGAAACGGAACTCTTTACTGAAGATGTTAAAAAACTGCTCGATGAGGATGAATACCATAAGCTTCAGGT
TTTTATGGCTCAGCATCCAGATTGTGGTGATGTCATTCAGGAAACGGGCGGCCTGAGAAAAATGCGCTGGGGATCGCGAG
GCAAAGGAAAGCGTAGTGGCGTGCGAATTATCTATTTTCACCGTAGTCAACGGTATGAGATTCGCTTGCTTCTGATTTAT
CAAAAAGGCATTAAAGATGATCTCACGCCGCAGGAAAAAGCGGTGCTTCGTATGCTGAATGAGAGGTGGTAG

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11521.99 Da
• Isoelectric Point: 9.4646

>AT206851 WP_000063156.1 NZ_CP077242:2376214-2376516 [Escherichia fergusonii]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Nucleotide

• Length: 303 bp

>AT206851 NZ_CP102719:226306-226596 [Salmonella enterica subsp. enterica serovar Kentucky]
ATGGATAAAGTGTTATTTGAGCGATTAACTCAAAGTATGTCTCAAATGAATGAAATCATTGAAGGGACCCGTAAGCCTTC
TCGTACCTTTCATATTGATGCAATGAAGATTAAAGAAATACGGCAGGCATCTGGGTTGTCGCAATCTAAGTTTGCAGAAC
TGATTTCGGTCAACGTGGATACGCTGCGCAACTGGGAGCAAGGAAGACGCTCACCGACAGGGCCAGCCAAAGCATTACTT
CGCGCCATTGCCAACGACCCGAGAAACGTTATACAAGCATTGCGTTATTGA

Structures

Antitoxin

• AlphaFold DB: A0A829G8Z0