Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 919238..919788 Replicon chromosome
Accession NZ_CP077242
Organism Escherichia fergusonii strain FDAARGOS 1438

Toxin (Protein)


Gene name ccdB Uniprot ID F0JPU1
Locus tag I6L68_RS04250 Protein ID WP_001160963.1
Coordinates 919238..919552 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag I6L68_RS04255 Protein ID WP_000125566.1
Coordinates 919555..919788 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6L68_RS04225 (914913) 914913..916199 + 1287 WP_000800490.1 peptidylprolyl isomerase SurA -
I6L68_RS04230 (916199) 916199..917188 + 990 WP_000241251.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
I6L68_RS04235 (917185) 917185..918006 + 822 WP_001065390.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
I6L68_RS04240 (918009) 918009..918386 + 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
I6L68_RS04245 (918393) 918393..919241 + 849 WP_000257179.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
I6L68_RS04250 (919238) 919238..919552 - 315 WP_001160963.1 CcdB family protein Toxin
I6L68_RS04255 (919555) 919555..919788 - 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
I6L68_RS04260 (919874) 919874..920353 - 480 WP_000624375.1 type 3 dihydrofolate reductase -
I6L68_RS04265 (920546) 920546..922408 - 1863 WP_000377099.1 glutathione-regulated potassium-efflux system protein KefC -
I6L68_RS04270 (922401) 922401..922931 - 531 WP_000600745.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
I6L68_RS04275 (923039) 923039..924370 - 1332 WP_001183186.1 MFS transporter -
I6L68_RS04280 (924429) 924429..924716 - 288 WP_000203741.1 ferredoxin-like protein FixX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11722.70 Da        Isoelectric Point: 8.0666

>T206846 WP_001160963.1 NZ_CP077242:c919552-919238 [Escherichia fergusonii]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSHIRPPERLNPILLLVDGKEYVLMTHETATVPVNTLG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T206846 NZ_CP102716:c207595-207335 [Lacticaseibacillus rhamnosus]
ATGATTAAAACCTGGACCGATGATGCTTGGGCGGACTACATGTATTGGCATGATCAAAACGACAAGCGGACAATCAAACG
AATTAATCAACTCATTCAAGCCATTGACCGTGACCCTTATAAAGGCATCGGAAAACCTGAGCCACTTAGATATGCGCTAA
CCGGAAAATGGTCACGTCGGATTGATCAGGAAAATCGCATCATCTACAGCATTGAAAAGAACCACATTAATATTTTCGCC
TGCCGCACTCACTACAGTTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT206846 WP_000125566.1 NZ_CP077242:c919788-919555 [Escherichia fergusonii]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT206846 NZ_CP102716:c207857-207588 [Lacticaseibacillus rhamnosus]
ATGGAAGCAACGAATTATAGTGATTTCCGCCGCAACCTTAAGCATTATATGAGTCAAGTCAACGAAGACGCCGAACCGCT
ACTGGTTACCGCTAAAGATGATGATGACAATGTGGTGGTTATGAGCAAGCACGATTTTGACGCCATCGAAGAAACCCTGT
ATTTACTCAGCAATCCCAAGCTGATGGCCAAAATCAAACGTGGTGATGCCCAAATTGCCGCTGGAAAGGCTAAACAGCAC
GAGTTGTTAACGGACTTCGATCATGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB F0JPU1

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References