Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 676873..677456 Replicon chromosome
Accession NZ_CP077242
Organism Escherichia fergusonii strain FDAARGOS 1438

Toxin (Protein)


Gene name mazF Uniprot ID V0SV58
Locus tag I6L68_RS03155 Protein ID WP_000254750.1
Coordinates 676873..677208 (-) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag I6L68_RS03160 Protein ID WP_000581937.1
Coordinates 677208..677456 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6L68_RS03140 (672760) 672760..674058 - 1299 WP_000036723.1 phosphopyruvate hydratase -
I6L68_RS03145 (674146) 674146..675783 - 1638 WP_000210873.1 CTP synthase (glutamine hydrolyzing) -
I6L68_RS03150 (676011) 676011..676802 - 792 WP_001071668.1 nucleoside triphosphate pyrophosphohydrolase -
I6L68_RS03155 (676873) 676873..677208 - 336 WP_000254750.1 endoribonuclease MazF Toxin
I6L68_RS03160 (677208) 677208..677456 - 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
I6L68_RS03165 (677534) 677534..679768 - 2235 WP_000226844.1 GTP diphosphokinase -
I6L68_RS03170 (679819) 679819..681117 - 1299 WP_000046872.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 671377..672705 1328


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12067.95 Da        Isoelectric Point: 8.2618

>T206845 WP_000254750.1 NZ_CP077242:c677208-676873 [Escherichia fergusonii]
MVSRYVPDTGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T206845 NZ_CP102712:c2687226-2686843 [Lacticaseibacillus rhamnosus]
ATGGATGTAACAGTCAAACGCGGGGATGTATTTTTCGCCGATTTATCGCCGGTTGTCGGGTCGGAACAAGGCGGCAACCG
GCCCGTACTTATTATTCAAAATAACGTTGGCAATCACTATAGTCCCACCGTCATTGTGGCTGCCATTACCTCCAAAATCC
AAAAACCCAAAATGCCCACTCATGTCGGTTTGCGCGCCAAGCAAGACGGGGTTGAGCGTAATTCAGTCATTTTGCTTGAA
CAGATTCGTACAATTGATAAGCAACGGTTACAGGCACGCGTTACCGCTCTTTCAAGTGCCAAAATGGCAGCGGTGGATCG
AGCACTGGCGATATCGGTCGGACTCGTCAGCTTGCCTAAGCCGAAAACATATAATAAAAACTAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT206845 WP_000581937.1 NZ_CP077242:c677456-677208 [Escherichia fergusonii]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT206845 NZ_CP102712:c2687494-2687246 [Lacticaseibacillus rhamnosus]
ATGAAGAAGGAAACGTCAGTAACGGTCGCGCTTGATAGTGCTATCAAAGCGCGTTTGGATGCCTATTGTGAGTTGAATGA
TATGGGTGTTCAGGATGTCATTACTGAAGCAGTGGCGAAGTTTTTGCAGTCACAAGCACCTGAGATCAATCAGTTGATTT
CGGGCTATGTGGCAATGGGACAAATCAATACGGAAATCAGCCATGCTTTCTCCGCCTGCGAAAGTGAGGCATACGCCCAT
ATTCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SV58

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References