206841

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) BrnTA/BrnT-BrnA
Location 4619..5204 Replicon plasmid unnamed2
Accession NZ_CP077240
Organism Acinetobacter pittii strain FDAARGOS 1399

Toxin (Protein)


Gene name brnT Uniprot ID A0A158LPL9
Locus tag I6L29_RS18920 Protein ID WP_002124950.1
Coordinates 4619..4906 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name brnA Uniprot ID A0A062GCE4
Locus tag I6L29_RS18925 Protein ID WP_005065064.1
Coordinates 4893..5204 (+) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6L29_RS18900 (I6L29_18905) 151..864 + 714 WP_005065071.1 hypothetical protein -
I6L29_RS18905 (I6L29_18910) 1562..2491 + 930 WP_005065049.1 replication initiation protein RepM -
I6L29_RS18910 (I6L29_18915) 2570..3100 + 531 WP_005065050.1 plasmid replication DNA-binding protein -
I6L29_RS18915 (I6L29_18920) 3427..4254 + 828 WP_052491491.1 hypothetical protein -
I6L29_RS18920 (I6L29_18925) 4619..4906 + 288 WP_002124950.1 BrnT family toxin Toxin
I6L29_RS18925 (I6L29_18930) 4893..5204 + 312 WP_005065064.1 BrnA antitoxin family protein Antitoxin
I6L29_RS18930 (I6L29_18935) 5295..5969 - 675 WP_005065062.1 HEPN domain-containing protein -
I6L29_RS18935 (I6L29_18940) 6021..6422 - 402 WP_005065059.1 hypothetical protein -
I6L29_RS18940 (I6L29_18945) 6514..6816 - 303 WP_005065057.1 XRE family transcriptional regulator -
I6L29_RS18945 (I6L29_18950) 6821..7165 - 345 WP_005065055.1 type II toxin-antitoxin system RelE/ParE family toxin -
I6L29_RS18950 (I6L29_18955) 7686..8003 - 318 WP_000900860.1 hypothetical protein -
I6L29_RS18955 (I6L29_18960) 8392..9276 - 885 WP_005065070.1 MobA/MobL family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..9581 9581


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(41-96)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11173.58 Da        Isoelectric Point: 4.9093

>T206841 WP_002124950.1 NZ_CP077240:4619-4906 [Acinetobacter pittii]
MELYFEWDEAKNQKNQKKHDVSFETASLVFEDPLRISIQDRHTNGEERWQTIGMVKGVLMLLVAHTIIDDDDCEIIRIIS
ARQVTKAERNQYEHG

Download         Length: 288 bp

>T206841 NZ_CP102697:c21219-20914 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCTGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGTTGTCAGATAAGGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAGGAAGTG
GCTGATCTCAGCCACCGAGAAAATGACATCAAAAATGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 12070.80 Da        Isoelectric Point: 9.4651

>AT206841 WP_005065064.1 NZ_CP077240:4893-5204 [Acinetobacter pittii]
MSMVRYTRKELNEKFSDKQDAEIKRLLAKGTVPDEQLDLSDIPEITDWSHAVRHGQFYRPVKQQTSVRLDADVLAWLKAQ
GKGYQTRMNKILREAMLEDLKNH

Download         Length: 312 bp

>AT206841 NZ_CP102697:c21439-21221 [Escherichia coli]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGGCT
GGTAAGCACCACCCTGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCAGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A158LPL9

Antitoxin

Source ID Structure
AlphaFold DB A0A062GCE4

References