Detailed information of TA system TA206825

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 1756336..1756952
• Replicon: chromosome
• Accession: NZ_CP077236
• Organism: Lelliottia amnigena strain FDAARGOS 1444

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A3S5XV95
• Locus tag: I6L74_RS08215
• Protein ID: WP_059180558.1
• Coordinates: 1756336..1756710 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: I6L74_RS08220
• Protein ID: WP_164841605.1
• Coordinates: 1756710..1756952 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6L74_RS08200 (I6L74_08200)	1753816..1754739	+	924	WP_015961045.1	formate dehydrogenase subunit beta	-
I6L74_RS08205 (I6L74_08205)	1754736..1755371	+	636	WP_059180560.1	formate dehydrogenase cytochrome b556 subunit	-
I6L74_RS08210 (I6L74_08210)	1755368..1756297	+	930	WP_059180559.1	formate dehydrogenase accessory protein FdhE	-
I6L74_RS08215 (I6L74_08215)	1756336..1756710	-	375	WP_059180558.1	type II toxin-antitoxin system VapC family toxin	Toxin
I6L74_RS08220 (I6L74_08220)	1756710..1756952	-	243	WP_164841605.1	CopG family transcriptional regulator	Antitoxin
I6L74_RS08225 (I6L74_08225)	1757158..1758099	-	942	WP_041689564.1	fatty acid biosynthesis protein FabY	-
I6L74_RS08230 (I6L74_08230)	1758144..1758581	-	438	WP_059180556.1	D-aminoacyl-tRNA deacylase	-
I6L74_RS08235 (I6L74_08235)	1758578..1759450	-	873	WP_059180555.1	virulence factor BrkB family protein	-
I6L74_RS08240 (I6L74_08240)	1759444..1760043	-	600	WP_015961052.1	glucose-1-phosphatase	-
I6L74_RS08245 (I6L74_08245)	1760176..1760976	-	801	WP_059180554.1	DeoR/GlpR family DNA-binding transcription regulator	-
I6L74_RS08250 (I6L74_08250)	1761011..1761907	-	897	WP_059180553.1	sugar kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-113)

Antitoxin

• Ribbon-helix-helix protein, CopG (13-47)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14003.14 Da
• Isoelectric Point: 7.4014

>T206825 WP_059180558.1 NZ_CP077236:c1756710-1756336 [Lelliottia amnigena]
MAKGSALFDTNILIDLFSGRAEAKHALESWPPQNAISIITWMEVMVGAKKYHQEHRTRIALSAFNVLGVSQEVAERSVDL
RQEYRMKLPDAIILATAQIHRLELVTRNTKDFGNIPDVVTPYNL

Nucleotide

• Length: 375 bp

>T206825 NZ_CP102694:c642396-641932 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGATGCTTTAGTTGC
CGAAGTCGAGGCATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGTAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9434.62 Da
• Isoelectric Point: 4.6034

>AT206825 WP_164841605.1 NZ_CP077236:c1756952-1756710 [Lelliottia amnigena]
MMAGRDMGRILLDLSDEVIKRLDDLKVHRNVPRAELLREAVEQYLERHDQAESTISDALGLWHGCEEEGVDYQRKLREEW

Nucleotide

• Length: 243 bp

>AT206825 NZ_CP102694:c642731-642396 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTCTTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: A0A3S5XV95