Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 5150112..5150732 Replicon chromosome
Accession NZ_CP077211
Organism Enterobacter cloacae strain FDAARGOS 1431

Toxin (Protein)


Gene name Hha Uniprot ID A0A5E1A1U8
Locus tag I6L61_RS25015 Protein ID WP_013095889.1
Coordinates 5150514..5150732 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID V3PBI9
Locus tag I6L61_RS25010 Protein ID WP_008499288.1
Coordinates 5150112..5150486 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6L61_RS25000 (I6L61_25000) 5145237..5146430 + 1194 WP_013095891.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
I6L61_RS25005 (I6L61_25005) 5146453..5149599 + 3147 WP_013095890.1 multidrug efflux RND transporter permease subunit AcrB -
I6L61_RS25010 (I6L61_25010) 5150112..5150486 + 375 WP_008499288.1 Hha toxicity modulator TomB Antitoxin
I6L61_RS25015 (I6L61_25015) 5150514..5150732 + 219 WP_013095889.1 HHA domain-containing protein Toxin
I6L61_RS25020 (I6L61_25020) 5150942..5151493 + 552 WP_013095888.1 maltose O-acetyltransferase -
I6L61_RS25025 (I6L61_25025) 5151609..5152076 + 468 WP_013095887.1 YlaC family protein -
I6L61_RS25030 (I6L61_25030) 5152048..5153508 - 1461 WP_013095886.1 PLP-dependent aminotransferase family protein -
I6L61_RS25035 (I6L61_25035) 5153610..5154320 + 711 WP_013095885.1 GNAT family protein -
I6L61_RS25040 (I6L61_25040) 5154317..5154457 - 141 WP_003859006.1 type B 50S ribosomal protein L36 -
I6L61_RS25045 (I6L61_25045) 5154460..5154720 - 261 WP_006176933.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-114)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8625.98 Da        Isoelectric Point: 8.9008

>T206810 WP_013095889.1 NZ_CP077211:5150514-5150732 [Enterobacter cloacae]
MSDKPLTKTDYLMRLRRCQSIDTLERVIEKNKYELSDNELTVFYSAADHRLAELTMNKLYDKIPPSVWKFVR

Download         Length: 219 bp

>T206810 NZ_CP102692:323081-323449 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTGGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14481.28 Da        Isoelectric Point: 4.8886

>AT206810 WP_008499288.1 NZ_CP077211:5150112-5150486 [Enterobacter cloacae]
MDEYSPKRHDIAQLKFLCESLYHDCLANLDESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFVNVSRANPVSLSC

Download         Length: 375 bp

>AT206810 NZ_CP102692:322863-323084 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5E1A1U8

Antitoxin

Source ID Structure
AlphaFold DB V3PBI9

References