Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 671517..672274 Replicon chromosome
Accession NZ_CP077211
Organism Enterobacter cloacae strain FDAARGOS 1431

Toxin (Protein)


Gene name tacT Uniprot ID A0A1D8JMF4
Locus tag I6L61_RS03165 Protein ID WP_044159004.1
Coordinates 671517..671999 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID A0A1D8JMH3
Locus tag I6L61_RS03170 Protein ID WP_007372584.1
Coordinates 671990..672274 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6L61_RS03145 (I6L61_03145) 666577..667857 - 1281 WP_176682058.1 type 4b pilus protein PilO2 -
I6L61_RS03150 (I6L61_03150) 667860..669533 - 1674 WP_013095157.1 PilN family type IVB pilus formation outer membrane protein -
I6L61_RS03155 (I6L61_03155) 669553..669996 - 444 WP_007372587.1 type IV pilus biogenesis protein PilM -
I6L61_RS03160 (I6L61_03160) 669998..671284 - 1287 WP_013095156.1 TcpQ domain-containing protein -
I6L61_RS03165 (I6L61_03165) 671517..671999 - 483 WP_044159004.1 GNAT family N-acetyltransferase Toxin
I6L61_RS03170 (I6L61_03170) 671990..672274 - 285 WP_007372584.1 DUF1778 domain-containing protein Antitoxin
I6L61_RS03175 (I6L61_03175) 672430..672930 - 501 WP_007372583.1 single-stranded DNA-binding protein -
I6L61_RS03180 (I6L61_03180) 673006..673497 - 492 WP_007372582.1 STY4534 family ICE replication protein -
I6L61_RS03185 (I6L61_03185) 674130..676142 - 2013 WP_044159002.1 DNA topoisomerase III -
I6L61_RS03190 (I6L61_03190) 676160..676882 - 723 WP_013095151.1 TIGR03761 family integrating conjugative element protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - - 575379..693081 117702


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(39-146)

Antitoxin

Vibrio phage ICP1, Orf50

(1-93)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17693.32 Da        Isoelectric Point: 6.9570

>T206794 WP_044159004.1 NZ_CP077211:c671999-671517 [Enterobacter cloacae]
MEINVTAPALLTEEHVLHSFDCGNAVLDDWLRRRALKNQHLNASRTFVICPEGTACVVGYYSLATGSVSHVDLSRSLRQN
MPDPVPVVLLGRLAVDVSTQGNNFGKWLLNDAVMRVSNLADQVGIKAIMVHAIDERAKAFYEYFGFVQSPVAANTLFYKI

Download         Length: 483 bp

>T206794 NZ_CP102682:480635-480883 [Leptospira borgpetersenii serovar Javanica]
ATGAAGGCTTTAGTTGTAAAAATAGGTAATTCAAAAGGAATTCGGATTCCCAAAGCGGTTTTAGAAGAATGCCATATTGA
AGAAGAAGTAGATTTACTAATCGATAACAATAAATTAATTATTGTGCCTCTAAAATCGAAACCAAGAGAAGGGTGGGAAA
AACAATTTAAATCAATGTCATCTAATAAAGATGATAAATTGCTTATTCCTGATTCAATAGATTTATCGGATAAGGATTGG
GAATGGTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 11078.63 Da        Isoelectric Point: 8.5684

>AT206794 WP_007372584.1 NZ_CP077211:c672274-671990 [Enterobacter cloacae]
MQTPVRKSVRDKQINIRATDEERAVIDYAASLVNKNRTDFIIERAVHEAQNIILDQRVFVLDDARYQAFIRQLEAPVQNV
EGRQRLMDVKPEWK

Download         Length: 285 bp

>AT206794 NZ_CP102682:480877-481200 [Leptospira borgpetersenii serovar Javanica]
ATGGTGATTTCTCAATACGAAGTATATTTGATAAATTTGGATCCAACGGTCGGACATGAGATTAAAAAATCAAGACCTTG
CGTAATAATCTCACCTAATGAAATGAATAAAACTATCGGAACAATTATTATTACACCAATGACTACAAAATCTCGATCAT
ATCCAACAAGAGTCGAATTAACATTTCAAGGAAAGAAAGGCTGGATTGTGTTAGATCAAATTCGAACTGTAGATAAAATA
CGTCTGATAAAAAAGTTAGGAAAAATAGATCCCAAAACAGTTAATAAAATGAAACTGATTATAAAGGAAATGTTGGTTGA
TTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1D8JMF4

Antitoxin

Source ID Structure
AlphaFold DB A0A1D8JMH3

References