Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 124108..125731 Replicon plasmid unnamed1
Accession NZ_CP077207
Organism Enterobacter bugandensis strain FDAARGOS 1427

Toxin (Protein)


Gene name hipA Uniprot ID C1IUI3
Locus tag I6L57_RS23030 Protein ID WP_000088044.1
Coordinates 124406..125731 (+) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A6M3PBR7
Locus tag I6L57_RS23025 Protein ID WP_012477377.1
Coordinates 124108..124401 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6L57_RS23005 (I6L57_23005) 119907..120299 + 393 WP_000341066.1 hypothetical protein -
I6L57_RS23010 (I6L57_23010) 120958..121614 + 657 WP_000462754.1 trypsin-like serine protease -
I6L57_RS23015 (I6L57_23015) 121817..122314 + 498 WP_000340139.1 membrane protein -
I6L57_RS23020 (I6L57_23020) 122319..123707 + 1389 WP_000083579.1 hypothetical protein -
I6L57_RS23025 (I6L57_23025) 124108..124401 + 294 WP_012477377.1 helix-turn-helix transcriptional regulator Antitoxin
I6L57_RS23030 (I6L57_23030) 124406..125731 + 1326 WP_000088044.1 type II toxin-antitoxin system HipA family toxin Toxin
I6L57_RS23035 (I6L57_23035) 125792..125998 + 207 WP_000134171.1 hypothetical protein -
I6L57_RS23040 (I6L57_23040) 126099..126509 + 411 WP_000985909.1 hypothetical protein -
I6L57_RS23045 (I6L57_23045) 126522..127337 + 816 WP_001641519.1 HNH endonuclease signature motif containing protein -
I6L57_RS23050 (I6L57_23050) 127590..128015 + 426 WP_000278471.1 hypothetical protein -
I6L57_RS23055 (I6L57_23055) 128564..128872 + 309 WP_001224686.1 hypothetical protein -
I6L57_RS23060 (I6L57_23060) 128888..129745 + 858 WP_000784387.1 HNH endonuclease signature motif containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(A) / dfrA14 / qnrB1 / aac(3)-IIa / blaOXA-1 / aac(6')-Ib-cr / blaCTX-M-15 / blaTEM-1B / aph(6)-Id / aph(3'')-Ib / sul2 / ant(3'')-Ia / catA1 - 1..298988 298988


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(8-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50333.32 Da        Isoelectric Point: 6.5044

>T206791 WP_000088044.1 NZ_CP077207:124406-125731 [Enterobacter bugandensis]
MSRKQQRLAIWMNGIKVGYWEKSKGVDSLQYLPDWSADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMRSSVENEWLCSVLLEQYGLPVARTQIAHFEDQKALVVERF
DRKWSGNGQWIIRLPQEDMCQALGISPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIVFWMMAATDGHAKNFS
ISIGPQGRYHLTPLYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

>T206791 NZ_CP102680:480648-480896 [Leptospira borgpetersenii serovar Javanica]
ATGAAGGCTTTAGTTGTAAAAATAGGTAATTCAAAAGGAATTCGGATTCCCAAAGCGGTTTTAGAAGAATGCCATATTGA
AGAAGAAGTAGATTTACTAATCGATAACAATAAATTAATTATTGTGCCTCTAAAATCGAAACCAAGAGAAGGGTGGGAAA
AACAATTTAAATCAATGTCATCTAATAAAGATGATAAATTGCTTATTCCTGATTCAATAGATTTATCGGATAAGGATTGG
GAATGGTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10860.30 Da        Isoelectric Point: 10.3134

>AT206791 WP_012477377.1 NZ_CP077207:124108-124401 [Enterobacter bugandensis]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSTTASSEGK
QTEEMLKSNSPARQEKW

Download         Length: 294 bp

>AT206791 NZ_CP102680:480890-481213 [Leptospira borgpetersenii serovar Javanica]
ATGGTGATTTCTCAATACGAAGTATATTTGATAAATTTGGATCCAACGGTCGGACATGAGATTAAAAAATCAAGACCTTG
CGTAATAATCTCACCTAATGAAATGAATAAAACTATCGGAACAATTATTATTACACCAATGACTACAAAATCTCGATCAT
ATCCAACAAGAGTCGAATTAACATTTCAAGGAAAGAAAGGCTGGATTGTGTTAGATCAAATTCGAACTGTAGATAAAATA
CGTCTGATAAAAAAGTTAGGAAAAATAGATCCCAAAACAGTTAATAAAATGAAACTGATTATAAAGGAAATGTTGGTTGA
TTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2S1XXW1

Antitoxin

Source ID Structure
AlphaFold DB A0A6M3PBR7

References