Detailed information of TA system TA206778

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 1088661..1089281
• Replicon: chromosome
• Accession: NZ_CP077206
• Organism: Enterobacter bugandensis strain FDAARGOS 1427

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: A0A1H8SI38
• Locus tag: I6L57_RS05145
• Protein ID: WP_008499287.1
• Coordinates: 1088661..1088879 (-)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: A0A8E1RNX1
• Locus tag: I6L57_RS05150
• Protein ID: WP_028012188.1
• Coordinates: 1088907..1089281 (-)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6L57_RS05115 (I6L57_05115)	1084672..1084932	+	261	WP_006176933.1	type B 50S ribosomal protein L31	-
I6L57_RS05120 (I6L57_05120)	1084935..1085075	+	141	WP_003859006.1	type B 50S ribosomal protein L36	-
I6L57_RS05125 (I6L57_05125)	1085072..1085782	-	711	WP_088205223.1	GNAT family protein	-
I6L57_RS05130 (I6L57_05130)	1085884..1087344	+	1461	WP_088205222.1	PLP-dependent aminotransferase family protein	-
I6L57_RS05135 (I6L57_05135)	1087316..1087783	-	468	WP_028012186.1	YlaC family protein	-
I6L57_RS05140 (I6L57_05140)	1087900..1088451	-	552	WP_028012187.1	maltose O-acetyltransferase	-
I6L57_RS05145 (I6L57_05145)	1088661..1088879	-	219	WP_008499287.1	HHA domain-containing protein	Toxin
I6L57_RS05150 (I6L57_05150)	1088907..1089281	-	375	WP_028012188.1	Hha toxicity modulator TomB	Antitoxin
I6L57_RS05155 (I6L57_05155)	1089791..1092937	-	3147	WP_047367620.1	multidrug efflux RND transporter permease subunit AcrB	-
I6L57_RS05160 (I6L57_05160)	1092960..1094153	-	1194	WP_028012190.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-114)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8612.00 Da
• Isoelectric Point: 8.9008

>T206778 WP_008499287.1 NZ_CP077206:c1088879-1088661 [Enterobacter bugandensis]
MSDKPLTKIDYLMRLRRCQSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFVR

Nucleotide

• Length: 219 bp

>T206778 NZ_CP102679:c2215373-2215197 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14511.30 Da
• Isoelectric Point: 4.8886

>AT206778 WP_028012188.1 NZ_CP077206:c1089281-1088907 [Enterobacter bugandensis]
MDEYSPKRHDIAQLKFLCESLYHDCLANLDESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINTQDLQKWRKSGNRLFRCFVNVSRANPVSLSC

Nucleotide

• Length: 375 bp

>AT206778 NZ_CP102679:c2215151-2214735 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• AlphaFold DB: A0A1H8SI38