Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09907-MazE
Location 14659..15260 Replicon plasmid pLPE-58K
Accession NZ_CP077102
Organism Lactiplantibacillus pentosus strain 9D3

Toxin (Protein)


Gene name pemK Uniprot ID Q684P1
Locus tag KSF55_RS16645 Protein ID WP_001748110.1
Coordinates 14916..15260 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A806J926
Locus tag KSF55_RS16640 Protein ID WP_001748109.1
Coordinates 14659..14922 (+) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KSF55_RS16600 (KSF55_16570) 10199..10471 + 273 WP_012491083.1 hypothetical protein -
KSF55_RS16605 (KSF55_16575) 10507..10974 + 468 WP_226769518.1 replication initiation protein RepA -
KSF55_RS16610 11199..11777 - 579 WP_056953173.1 hypothetical protein -
KSF55_RS16615 11827..12504 - 678 Protein_13 relaxase/mobilization nuclease domain-containing protein -
KSF55_RS16620 12486..12839 - 354 WP_021730116.1 plasmid mobilization relaxosome protein MobC -
KSF55_RS16625 (KSF55_16590) 13307..13576 - 270 WP_001748276.1 helix-turn-helix transcriptional regulator -
KSF55_RS16630 (KSF55_16595) 13563..13934 - 372 WP_001748275.1 type II toxin-antitoxin system RelE/ParE family toxin -
KSF55_RS16635 (KSF55_16600) 13986..14573 - 588 WP_252270488.1 site-specific integrase -
KSF55_RS16640 (KSF55_16605) 14659..14922 + 264 WP_001748109.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
KSF55_RS16645 (KSF55_16610) 14916..15260 + 345 WP_001748110.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
KSF55_RS16650 (KSF55_16615) 15803..16270 + 468 WP_003587210.1 DNA starvation/stationary phase protection protein -
KSF55_RS16655 (KSF55_16620) 16517..17260 + 744 WP_003581836.1 ABC transporter ATP-binding protein -
KSF55_RS16660 (KSF55_16625) 17238..18485 + 1248 WP_027822874.1 ABC transporter permease -
KSF55_RS16665 (KSF55_16630) 18490..19161 + 672 WP_025025373.1 TetR/AcrR family transcriptional regulator -
KSF55_RS16670 (KSF55_16635) 19265..19414 - 150 WP_225364958.1 hypothetical protein -
KSF55_RS16675 (KSF55_16640) 19431..20101 - 671 Protein_25 IS5 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..58157 58157


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-104)

Antitoxin

Antitoxin PemI-like

(8-85)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 13041.89 Da        Isoelectric Point: 6.4782

>T206760 WP_001748110.1 NZ_CP077102:14916-15260 [Lactiplantibacillus pentosus]
MVSQGDIFYVNFNPSRGHEQMNKRPAIALSNDLVCQTSNMTIVAPISSTKRNFPMYHRLTSSQTVYGKVLLDQTIALDLR
ARHVTDETIVDHVSREELEEIITLYKLLFSIDDK

Download         Length: 345 bp

>T206760 NZ_CP102675:c4724364-4723987 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCCCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGGAAGCATCCGGAGGCGAAACGATGA

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 10141.47 Da        Isoelectric Point: 5.7117

>AT206760 WP_001748109.1 NZ_CP077102:14659-14922 [Lactiplantibacillus pentosus]
MELIKEQTRLAKWGNSKAARIPSQIIKQLKLDDNQDMTITIENGSIVLTPIKKNPTNIHELFKDWQDDGKRDHELDWGKS
EGNELQW

Download         Length: 264 bp

>AT206760 NZ_CP102675:c4724822-4724454 [Escherichia coli]
GTGTCAGACGCACTCTCCGGGACAATGCTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGTCTGACCTGCAAAGCTGACACTCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0R2K1X3

Antitoxin

Source ID Structure
AlphaFold DB A0A806J926

References