Detailed information of TA system TA206754

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2239017..2239546
• Replicon: chromosome
• Accession: NZ_CP077098
• Organism: Staphylococcus aureus strain RGB-095930

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: KSF82_RS11390
• Protein ID: WP_000621175.1
• Coordinates: 2239017..2239379 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: KSF82_RS11395
• Protein ID: WP_000948331.1
• Coordinates: 2239376..2239546 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KSF82_RS11370 (2235995)	2235995..2236765	-	771	WP_001041103.1	RNA polymerase sigma factor SigB	-
KSF82_RS11375 (2236740)	2236740..2237219	-	480	WP_220668425.1	anti-sigma B factor RsbW	-
KSF82_RS11380 (2237221)	2237221..2237547	-	327	WP_001052491.1	anti-sigma factor antagonist	-
KSF82_RS11385 (2237666)	2237666..2238667	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
KSF82_RS11390 (2239017)	2239017..2239379	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
KSF82_RS11395 (2239376)	2239376..2239546	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
KSF82_RS11400 (2239631)	2239631..2240779	-	1149	WP_001281145.1	alanine racemase	-
KSF82_RS11405 (2240845)	2240845..2241204	-	360	WP_000581200.1	holo-ACP synthase	-
KSF82_RS11410 (2241208)	2241208..2241699	-	492	WP_001205910.1	PH domain-containing protein	-
KSF82_RS11415 (2241686)	2241686..2243269	-	1584	WP_220668426.1	PH domain-containing protein	-
KSF82_RS11420 (2243262)	2243262..2243741	-	480	WP_001287088.1	hypothetical protein	-
KSF82_RS11425 (2243949)	2243949..2244509	-	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T206754 WP_000621175.1 NZ_CP077098:c2239379-2239017 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T206754 NZ_CP102675:c3586109-3585732 [Escherichia coli]
ATGAAAACATTACCTGACACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGTTGGACCAGCATTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCAGGATTC
AGCGCCTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCCCGCCGGGCAACCGGCCTGATGGCCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT206754 WP_000948331.1 NZ_CP077098:c2239546-2239376 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT206754 NZ_CP102675:c3586530-3586156 [Escherichia coli]
GTGTCAGACACTCTCCCCGGGACAACGCATCCCGACGATAACAACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTTGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATATGGCTGTTTATCCGACACTGGCACCCGCAACCACCTCATAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1