Detailed information of TA system TA206743

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/upstrm_HI1419-dnstrm_HI1420
• Location: 3822918..3823527
• Replicon: chromosome
• Accession: NZ_CP077097
• Organism: Pseudomonas anuradhapurensis strain RD8MR3

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: HU763_RS17600
• Protein ID: WP_186688536.1
• Coordinates: 3822918..3823220 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: HU763_RS17605
• Protein ID: WP_186688533.1
• Coordinates: 3823213..3823527 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HU763_RS24860	3818832..3818960	-	129	WP_009683677.1	PA1414 family protein	-
HU763_RS17580 (HU763_017580)	3819085..3819978	-	894	WP_186688545.1	LysR family transcriptional regulator	-
HU763_RS17585 (HU763_017585)	3820084..3821253	+	1170	WP_186688543.1	MFS transporter	-
HU763_RS17590 (HU763_017590)	3821314..3822240	-	927	WP_186688541.1	DMT family transporter	-
HU763_RS17595 (HU763_017595)	3822347..3822742	-	396	WP_186688539.1	transcriptional regulator	-
HU763_RS17600 (HU763_017600)	3822918..3823220	+	303	WP_186688536.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HU763_RS17605 (HU763_017605)	3823213..3823527	+	315	WP_186688533.1	putative addiction module antidote protein	Antitoxin
HU763_RS17610 (HU763_017610)	3823579..3823851	+	273	WP_186688530.1	hypothetical protein	-
HU763_RS17615 (HU763_017615)	3824019..3825380	-	1362	WP_186688527.1	DEAD/DEAH box helicase	-
HU763_RS17620 (HU763_017620)	3825484..3826785	+	1302	WP_186688524.1	mechanosensitive ion channel family protein	-
HU763_RS17625 (HU763_017625)	3826820..3828208	-	1389	WP_186688521.1	cytosine permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3815100..3834751	19651

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (6-94)

Antitoxin

• Uncharacterized protein HI1420 (8-97)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11378.01 Da
• Isoelectric Point: 10.8773

>T206743 WP_186688536.1 NZ_CP077097:3822918-3823220 [Pseudomonas anuradhapurensis]
MKKIESSSFRHWVTGLRDVNARTRIISRINRLMEGLPGDVSPVGHGVSELRVHYGPGYRVYFHQAGSTFVILLCGGDKGS
QQRDIKTAHQILRSWRMQND

Nucleotide

• Length: 303 bp

>T206743 NZ_CP102675:1368293-1368511 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11306.83 Da
• Isoelectric Point: 6.2193

>AT206743 WP_186688533.1 NZ_CP077097:3823213-3823527 [Pseudomonas anuradhapurensis]
MTETFSEFDPAQYLTTPEAIAQFMTDALETGDAGYVAKAVGVVARAKGMSELAKETGLSREQLYRSFSEHGNPTLKSFLT
VMKALGIDMTAQPHLAGHRSRPLN

Nucleotide

• Length: 315 bp

>AT206743 NZ_CP102675:1367893-1368267 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG