Detailed information of TA system TA206742

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 2190435..2191318
• Replicon: chromosome
• Accession: NZ_CP077097
• Organism: Pseudomonas anuradhapurensis strain RD8MR3

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: HU763_RS10140
• Protein ID: WP_186684593.1
• Coordinates: 2190881..2191318 (+)
• Length: 146 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: HU763_RS10135
• Protein ID: WP_186684591.1
• Coordinates: 2190435..2190884 (+)
• Length: 150 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HU763_RS10110 (HU763_010110)	2185858..2187057	+	1200	WP_186684580.1	sugar transporter	-
HU763_RS10115 (HU763_010115)	2187109..2187693	-	585	WP_186684582.1	LysE family transporter	-
HU763_RS10120 (HU763_010120)	2187744..2188574	-	831	WP_186684584.1	AraC family transcriptional regulator	-
HU763_RS10125 (HU763_010125)	2188624..2189547	-	924	WP_186684586.1	LysR family transcriptional regulator	-
HU763_RS10130 (HU763_010130)	2189652..2190305	+	654	WP_186684588.1	oxygen-insensitive NAD(P)H nitroreductase	-
HU763_RS10135 (HU763_010135)	2190435..2190884	+	450	WP_186684591.1	DUF2384 domain-containing protein	Antitoxin
HU763_RS10140 (HU763_010140)	2190881..2191318	+	438	WP_186684593.1	RES family NAD+ phosphorylase	Toxin
HU763_RS10145 (HU763_010145)	2191326..2192480	-	1155	WP_186684595.1	aminotransferase class V-fold PLP-dependent enzyme	-
HU763_RS10150 (HU763_010150)	2192675..2193601	-	927	WP_170029332.1	LysR family transcriptional regulator	-
HU763_RS10155 (HU763_010155)	2193742..2194965	+	1224	WP_186684598.1	acyl-CoA dehydrogenase	-
HU763_RS10160 (HU763_010160)	2194962..2195771	-	810	WP_186685055.1	OBAP family protein	-
HU763_RS10165 (HU763_010165)	2195926..2196294	+	369	WP_170029334.1	DNA binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-137)

Antitoxin

• Antitoxin Xre-like, helix-turn-helix domain (33-91)

Sequences

Toxin

Protein

• Length: 146 a.a.
• Molecular weight: 16059.36 Da
• Isoelectric Point: 6.0718

>T206742 WP_186684593.1 NZ_CP077097:2190881-2191318 [Pseudomonas anuradhapurensis]
VILWRISAYADLSGTGGLRVSGRWHQAGRPVVYAATSPAGAMLEVLVHLEIDPEDFPTTMRLLRIELPDTVSQAQLPALQ
PGWSTQTELTRGLGNRFLDDCSALLLPVPSAIMPRTINYLFNPRHPQAHSARLDVEDFTPDSRLF

Nucleotide

• Length: 438 bp

>T206742 NZ_CP102675:612043-612150 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin

Protein

• Length: 150 a.a.
• Molecular weight: 17002.62 Da
• Isoelectric Point: 6.3398

>AT206742 WP_186684591.1 NZ_CP077097:2190435-2190884 [Pseudomonas anuradhapurensis]
MLAEVLRDNGYHEYRVRLHALLEIPELASDFEIHTRITHGFAATWLVKLTELGVFTPVERDQIIPLRTLKSRIERDQPLT
VEESDRLFRSAHITAMAEAVFGDAAKAKRWLSKPKERFAGLTPMQMLTTQQGTTQVEEMLLQIAEGFGL

Nucleotide

• Length: 450 bp

>AT206742 NZ_CP102675:c611993-611930 [Escherichia coli]
TCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTAAACCTCTCAACGTGCGGGGGTTTTCT