Detailed information of TA system TA206727

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc(toxin)
• Location: 5100365..5100939
• Replicon: chromosome
• Accession: NZ_CP077092
• Organism: Pseudomonas iranensis strain SWRI54

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: HU724_RS22855
• Protein ID: WP_122747781.1
• Coordinates: 5100365..5100769 (-)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: A0A6I0CH28
• Locus tag: HU724_RS22860
• Protein ID: WP_073473286.1
• Coordinates: 5100769..5100939 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HU724_RS22840 (HU724_022840)	5095817..5097463	-	1647	WP_186569498.1	alpha,alpha-phosphotrehalase	-
HU724_RS22845 (HU724_022845)	5097580..5099022	-	1443	WP_137214094.1	PTS system trehalose-specific EIIBC component	-
HU724_RS22850 (HU724_022850)	5099257..5099961	+	705	WP_016773346.1	trehalose operon repressor	-
HU724_RS22855 (HU724_022855)	5100365..5100769	-	405	WP_122747781.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
HU724_RS22860 (HU724_022860)	5100769..5100939	-	171	WP_073473286.1	acetyltransferase	Antitoxin
HU724_RS22865 (HU724_022865)	5101153..5102730	-	1578	WP_123444230.1	glutamine-hydrolyzing GMP synthase	-
HU724_RS22870 (HU724_022870)	5102811..5104280	-	1470	WP_016773343.1	IMP dehydrogenase	-
HU724_RS22875 (HU724_022875)	5104386..5104934	-	549	WP_186569499.1	sugar ABC transporter ATPase	-
HU724_RS22880 (HU724_022880)	5105070..5105837	-	768	WP_186569500.1	sulfite exporter TauE/SafE family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (9-121)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 14773.64 Da
• Isoelectric Point: 4.1858

>T206727 WP_122747781.1 NZ_CP077092:c5100769-5100365 [Pseudomonas iranensis]
MELIHFPFERVIEINTQILTTEPGMKGRVNIPILQGALARIDNAIAYNGLDDVFEIAAKYTASIAQGHALPDANKRTGLA
VGLEYLSLNDYEITLDNDLLADAVRDLVTGEINEHDFADILYSCFKASQNQGSY

Nucleotide

• Length: 405 bp

>T206727 NZ_CP102669:c4315381-4315097 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGACTTATGAACTGGAATTCGACCCGAGGGCCTTAAAAGAGTGGCATAAGCTGGGCGATACGGTGAAGGCTCAGCTTAA
GAAAAAGTTGGCTGATGTGCTATTGAACCCCAGAATCGACTCTGCCCGTTTAAACGGTCTTCCTGACTGCTATAAAATTA
AGCTTAAATCGTCCGGTTATCGTCTGGTGTACCAGGTTCGGGATGACGTTGTGATTGTGTTTGTTGTTGCGGTCGGCAAG
CGAGAACATTCAGCCGTCTATCACGATGCAAACAAACGGCTTTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6375.56 Da
• Isoelectric Point: 10.5359

>AT206727 WP_073473286.1 NZ_CP077092:c5100939-5100769 [Pseudomonas iranensis]
MNHKLSTYGVCIVDRPKIKATKKLDLGGDQGKQIVYSETKLVLRTHQKTFKKLADM

Nucleotide

• Length: 171 bp

>AT206727 NZ_CP102669:c4315613-4315371 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTGAACATTACTCC
AACGGAAGCCGTGCGATTACTGTTCCAGTACGTCGCGGAAACGGGGCGCATGCCAGTGAAAACCGTCACTCTTAGCGACA
GTGAAGATGCATTGATTCAGACAGTTCGGGAGAGGCTGTCTAGTCCGCAAAAGGGAATCAAGGTTAGTCTGGATGACTTA
TGA

Structures

Antitoxin

• AlphaFold DB: A0A6I0CH28