Detailed information of TA system TA206720

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 894871..895467
• Replicon: chromosome
• Accession: NZ_CP077092
• Organism: Pseudomonas iranensis strain SWRI54

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: HU724_RS03920
• Protein ID: WP_186567314.1
• Coordinates: 895165..895467 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: J2PUL8
• Locus tag: HU724_RS03915
• Protein ID: WP_007914567.1
• Coordinates: 894871..895161 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HU724_RS03895 (HU724_003895)	891173..893293	-	2121	WP_186567320.1	TonB-dependent copper receptor	-
HU724_RS03900 (HU724_003900)	893378..893824	-	447	WP_133337097.1	DUF2946 domain-containing protein	-
HU724_RS03905 (HU724_003905)	893846..894325	-	480	WP_186567318.1	copper chaperone PCu(A)C	-
HU724_RS03910 (HU724_003910)	894374..894772	-	399	WP_186567316.1	DUF2946 domain-containing protein	-
HU724_RS03915 (HU724_003915)	894871..895161	-	291	WP_007914567.1	putative addiction module antidote protein	Antitoxin
HU724_RS03920 (HU724_003920)	895165..895467	-	303	WP_186567314.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HU724_RS03925 (HU724_003925)	895626..896354	-	729	WP_186567312.1	cobalt-precorrin-6A reductase	-
HU724_RS03930 (HU724_003930)	896351..897448	-	1098	WP_186567310.1	cobalt-precorrin-5B (C(1))-methyltransferase	-
HU724_RS03935 (HU724_003935)	897441..898652	-	1212	WP_186567308.1	precorrin-6y C5,15-methyltransferase (decarboxylating) subunit CbiE	-
HU724_RS03940 (HU724_003940)	898785..900083	+	1299	WP_275970775.1	precorrin-3B synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-96)

Antitoxin

• Uncharacterized protein HI1420 (6-95)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11468.17 Da
• Isoelectric Point: 9.7106

>T206720 WP_186567314.1 NZ_CP077092:c895467-895165 [Pseudomonas iranensis]
MIAFERSRIFSEWLDSLKDIKGKGRIISRIKTAENGNFGDCGFVGDTVYEMRVHYGPGYRMYFTRKGEVIYLLLIGGDKS
TQKRDIKRAVQMAHSIGNEE

Nucleotide

• Length: 303 bp

>T206720 NZ_CP102669:844026-844424 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGCTGAAATTCATGCTTGATACCAATACCTGTATTTTCACCATCAAAAATAAGCCCGAACACATCAGAGAACGCTTCAA
CCTCAATACATCCCGAATGTGTATCAGCTCCATCACCTTAATGGAGCTGATTTACGGTGCTGAAAAAAGCCTGGCGCCGG
AGCGTAATCTTGCCGTCGTGGAGGGATTTATCTCCCGCCTTGAGGTTTTGGATTACGATACACAGGCAGCGATACATACC
GGTCAAATCCGTGCCGAACTGGCCCGCAAGGGAACACCTGTCGGGCCTTATGACCAGATGATTGCTGGCCATGCCGGTAG
CCGCGGACTGGTCGTCGTCACAAACAATCTCCGCGAATTTGAACGCATTCCGGGTATCCGAATCGAAGACTGGTGCTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10445.87 Da
• Isoelectric Point: 4.3525

>AT206720 WP_007914567.1 NZ_CP077092:c895161-894871 [Pseudomonas iranensis]
MTEEFARFDAAEYLKTPEDMAAYLDACFDDDEGDGVLIRAALNDIARAQGMTQVARDAGLGRESLYKALSSTGNPEFATI
MKVMKALGLRLHAVTL

Nucleotide

• Length: 291 bp

>AT206720 NZ_CP102669:843799-844026 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGCACACAACACTTTTTTTTAGTAACCGGACCCAGGCAGTCAGATTGCCTAAATCGATATCCTTTCCGGAGGATGTGAA
ACATGTCGAAATCATTGCGGTTGGCAGAAGCAGAATCATTACTCCAGTCGGAGAATCCTGGGACAGCTGGTTCGATGGCG
AAGGCGCCAGTACTGATTTTATGAGTACCAGAGAACAACCCGCAGTACAGGAACGGGAAGGATTTTGA

Structures

Antitoxin

• AlphaFold DB: J2PUL8