Detailed information of TA system TA206712

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 4174907..4176531
• Replicon: chromosome
• Accession: NZ_CP077091
• Organism: Pseudomonas hamedanensis strain SWRI65

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: HU739_RS18060
• Protein ID: WP_186546153.1
• Coordinates: 4174907..4176256 (+)
• Length: 450 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: HU739_RS18065
• Protein ID: WP_186546152.1
• Coordinates: 4176253..4176531 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HU739_RS18050 (HU739_018050)	4170348..4172156	+	1809	WP_186546155.1	sodium-extruding oxaloacetate decarboxylase subunit alpha	-
HU739_RS18055 (HU739_018055)	4172354..4174543	-	2190	WP_186546154.1	autotransporter outer membrane beta-barrel domain-containing protein	-
HU739_RS18060 (HU739_018060)	4174907..4176256	+	1350	WP_186546153.1	HipA domain-containing protein	Toxin
HU739_RS18065 (HU739_018065)	4176253..4176531	+	279	WP_186546152.1	helix-turn-helix transcriptional regulator	Antitoxin
HU739_RS18070 (HU739_018070)	4176547..4177383	-	837	WP_186546151.1	amino acid ABC transporter permease	-
HU739_RS18075 (HU739_018075)	4177380..4178201	-	822	WP_186546150.1	ABC transporter substrate-binding protein	-
HU739_RS18080 (HU739_018080)	4178315..4179217	+	903	WP_186546149.1	homocysteine S-methyltransferase family protein	-
HU739_RS18085 (HU739_018085)	4179343..4180014	-	672	WP_186546148.1	GNAT family protein	-
HU739_RS18090 (HU739_018090)	4180025..4180471	-	447	WP_186546147.1	GNAT family N-acetyltransferase	-
HU739_RS18095 (HU739_018095)	4180484..4181107	-	624	WP_186546146.1	FMN-binding negative transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (165-367)

Antitoxin

• Cro/C1-type helix-turn-helix domain (27-80)

Sequences

Toxin

Protein

• Length: 450 a.a.
• Molecular weight: 49963.66 Da
• Isoelectric Point: 5.5076

>T206712 WP_186546153.1 NZ_CP077091:4174907-4176256 [Pseudomonas hamedanensis]
MHKLTLQIFTAGYWQDAMVLNFDDPEKGFESRCSFGYEAGYLLNNIESIGSPFSKAVSALYPLDWDGQRSKTPAFVHDIA
PAGAAKRFLLARLGQERPAEISPDLFLLGRRTPAPIGNMRIKESAEAVDERRPIGFKRAEVIHRDNRFLEYAYEQGAAIG
GATGAGGEAPKLLLAQNHAGLLYPDAVLDDVDVQQHWFVKFARNKGGQTDQDILRSEFHYYKALQILGIETVAAEGLALE
EANKPSLWMQRFDREVKDGAVSRYAVESIYSLAGVTTPGSAMNHLEVIRMLAGFWRQAGQASQIPALVADYLRRDLINKI
LGNSDNHGRNTAIIRRENAFQLAPIYDLAPMVMDDEGVTRTTKWPKELERAGDVDWRGVCRILADSADPDEPLAVLDASE
LFDELRADARRLAALPDILAASGLPARTMNHPGIHLKNLEQRLKEWDLQ

Nucleotide

• Length: 1350 bp

>T206712 NZ_CP102665:201870-202163 [Sphingobium sp. JS3065]
ATGAAAATCCAATGGACCAGCAAAGCGTCATCGGATCTTTTACGCCTGCACGAACATCTTCGACCAGTCGCGCCCGAGGC
AGCCGCTCGCGTCGTACAGCAACTCGCCCATGCGCCTAACCGCCTGCTCGACTATCCCCGCCTCGGCGAGAAACTGGAAG
CCTTCGAGCCGCGTGAGGTTCGGCGGATCATCGTGGGCAACTATGAAATGCGATACGAGATCGCGAGCGGCACCATCTTC
ATCCTGCGCCTCTGGCACTGCCGCGAGAACCGGAGCTTCGAGTCCGAGCATTGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10373.13 Da
• Isoelectric Point: 10.9383

>AT206712 WP_186546152.1 NZ_CP077091:4176253-4176531 [Pseudomonas hamedanensis]
MSLTVLERTALIESIQESLAQGTLEIGDAVRRLRVEVTGLHQTQFARMCKISVRTLVHIEHGEGNQTLKSLNAVFRPFGM
KMGVVRIRRDNL

Nucleotide

• Length: 279 bp

>AT206712 NZ_CP102665:201604-201873 [Sphingobium sp. JS3065]
ATGGCGGCAGCAACGAGAGTCCTAACGGCCCATGTGCCGGAGGGCTTGGCCGATAAAGTGGATGCTCTGGCAGCACGCAT
CGAGCGCTCACGAGGCTGGATCATGAAGCAAGCCTTGGCGGCATGGATCGATCAGGAGGAAGAGCGGCACAAGATGACGC
TCGAAGCTCTGGCCGACGTCGATGCGGGCCGGGTGATCGATCATCAGAGCATCCAGGCATGGGCAGACAGCCTGTCCACG
GATAGCCCGTTACCCTCGCCGACCGCATGA