Detailed information of TA system TA206710

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-higA/HigB-HigA
• Location: 3731773..3732652
• Replicon: chromosome
• Accession: NZ_CP077091
• Organism: Pseudomonas hamedanensis strain SWRI65

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: HU739_RS16125
• Protein ID: WP_186548483.1
• Coordinates: 3732299..3732652 (-)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HU739_RS16120
• Protein ID: WP_225922739.1
• Coordinates: 3731773..3732273 (-)
• Length: 167 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HU739_RS16110 (HU739_016110)	3727000..3728925	-	1926	WP_186548479.1	ATP-dependent helicase	-
HU739_RS16115 (HU739_016115)	3728910..3731048	-	2139	WP_186548481.1	ATP-dependent endonuclease	-
HU739_RS16120 (HU739_016120)	3731773..3732273	-	501	WP_225922739.1	transcriptional regulator	Antitoxin
HU739_RS16125 (HU739_016125)	3732299..3732652	-	354	WP_186548483.1	type II toxin-antitoxin system HigB family toxin	Toxin
HU739_RS16130 (HU739_016130)	3733173..3733598	-	426	WP_186548485.1	type II toxin-antitoxin system HicB family antitoxin	-
HU739_RS16135 (HU739_016135)	3733630..3733812	-	183	WP_186548487.1	type II toxin-antitoxin system HicA family toxin	-
HU739_RS16140 (HU739_016140)	3734283..3734564	+	282	WP_186548489.1	DUF3077 domain-containing protein	-
HU739_RS16145 (HU739_016145)	3734609..3735289	-	681	WP_225922740.1	RES family NAD+ phosphorylase	-
HU739_RS16150 (HU739_016150)	3735286..3735693	-	408	WP_186548491.1	MbcA/ParS/Xre antitoxin family protein	-
HU739_RS16155 (HU739_016155)	3736014..3736940	+	927	WP_186548493.1	LysR family transcriptional regulator	-
HU739_RS16160 (HU739_016160)	3736857..3737174	-	318	WP_024011198.1	AzlD domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3714324..3734564	20240

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (74-111)

Antitoxin

• Antitoxin HigA (52-163)

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 13823.01 Da
• Isoelectric Point: 9.8453

>T206710 WP_186548483.1 NZ_CP077091:c3732652-3732299 [Pseudomonas hamedanensis]
MRVIAKTAVAEAIEKYSQWKDPLTLWLSTFDRPPLRFESFEQIREVWVNTSGWNVDRIPRSKLRLPSQKGPLDIYVFDIK
KNECRIIVWINTRNGTIFIKDILPHAGYDKWCNSDVK

Nucleotide

• Length: 354 bp

>T206710 NZ_CP102664:c889455-889159 [Sphingobium sp. JS3065]
ATGGCCTATAGACTCACGCGTAACGCCGAAAAGGATCTCATCGGTATATATGTCGGCGGCGTCGGAGAATTAGGAGAAGC
CGTCGCGGAAAAATATCAGGCCGGTTTGCATCGGGTTTTTGGATTTCTCTCCGACTTTCCCTATTCGGCAAGAGCGCGCG
CGGAAATTTCACCACCGGTCCGGGCACATCCCTACAAGTCCCATATCGTCGTCTATATCATCGAAGGACAGGACATCCTG
ATCCTCGGCGTCCGTCATGGACGGGAAGACTGGCAGGCATCCGACCAGCTTTCCTGA

Antitoxin

Protein

• Length: 167 a.a.
• Molecular weight: 18674.14 Da
• Isoelectric Point: 4.3494

>AT206710 WP_225922739.1 NZ_CP077091:c3732273-3731773 [Pseudomonas hamedanensis]
MYYEIIGDEMKRDKIEATGLETFIANISPMFDSMSDQIATVSKLLSACHDPIESDDDLRGRMTLIDELYSHADSEDHAAA
RFAELVADRVYEYETATVLIPYSSQSEALAFLMNDRGIKQKDLADIASQSAISEILNNKRKMTVAQIKGFSDFFKVPVEF
FMHGVV

Nucleotide

• Length: 501 bp

>AT206710 NZ_CP102664:c889696-889445 [Sphingobium sp. JS3065]
ATGGCGACAATGAATGTTTCCCTCCCCGATGCGATGAAGGAATGGGTCGAAGGTCAGGCCGGGACAGGCAGATACAGCAA
TGCCAGCGATTATGTGCGCGACCTGATCCGTCGCGACCAGGAGCGGCGGGAGAAGATCGCAGCCATGCAGACCATGGTCG
ATGAGTCTCTCGCCTCCGGCATAAGTCCCAATTCCATCGATGACATGATGAAGGAAGCCCGGCGTCGCGCAGGCGTCGGT
CATGGCCTATAG