Detailed information of TA system TA206709

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 3714094..3714731
• Replicon: chromosome
• Accession: NZ_CP077091
• Organism: Pseudomonas hamedanensis strain SWRI65

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: HU739_RS16045
• Protein ID: WP_186548463.1
• Coordinates: 3714324..3714731 (+)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A9E6NX64
• Locus tag: HU739_RS16040
• Protein ID: WP_039756568.1
• Coordinates: 3714094..3714324 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HU739_RS15995 (HU739_015995)	3709504..3710571	+	1068	WP_186548447.1	A/G-specific adenine glycosylase	-
HU739_RS16000 (HU739_016000)	3710568..3710840	+	273	WP_186548449.1	oxidative damage protection protein	-
HU739_RS16010 (HU739_016010)	3711103..3711450	+	348	WP_186548451.1	hypothetical protein	-
HU739_RS16015 (HU739_016015)	3711463..3711753	-	291	WP_186548453.1	YceK/YidQ family lipoprotein	-
HU739_RS16020 (HU739_016020)	3711847..3712053	-	207	WP_186548455.1	hypothetical protein	-
HU739_RS16025 (HU739_016025)	3712086..3713027	+	942	WP_186548457.1	alpha/beta fold hydrolase	-
HU739_RS16030 (HU739_016030)	3713057..3713470	+	414	WP_186548459.1	hypothetical protein	-
HU739_RS16035 (HU739_016035)	3713477..3713929	+	453	WP_186548461.1	hypothetical protein	-
HU739_RS16040 (HU739_016040)	3714094..3714324	+	231	WP_039756568.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
HU739_RS16045 (HU739_016045)	3714324..3714731	+	408	WP_186548463.1	tRNA(fMet)-specific endonuclease VapC	Toxin
HU739_RS16050 (HU739_016050)	3714802..3716193	-	1392	WP_186548465.1	GABA permease	-
HU739_RS16055 (HU739_016055)	3716748..3717521	+	774	WP_025110949.1	ABC transporter ATP-binding protein	-
HU739_RS16060 (HU739_016060)	3717535..3718284	+	750	WP_186548467.1	ABC transporter substrate-binding protein	-
HU739_RS16065 (HU739_016065)	3718363..3719058	+	696	WP_186548469.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-124)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 14954.31 Da
• Isoelectric Point: 5.7398

>T206709 WP_186548463.1 NZ_CP077091:3714324-3714731 [Pseudomonas hamedanensis]
MIKYMLDTNICIFTIKNKPQVVREAFNRHSGQLCVSAITLMELIYGAEKSAAPEKNLAIVEGFVARLEVLPFDNDAAAQT
GMIRSELAKAGTPIGPYDQMIAGHARSLGLIVVTNNVREFERVSGLRIEDWVYPD

Nucleotide

• Length: 408 bp

>T206709 NZ_CP102664:c129794-129636 [Sphingobium sp. JS3065]
GTGAAACGCCTCGCGGATGAAGGCTTTGAACTGGTTTCGGTGAAAGGCTCTCACCACAAGTTCCGCAAGGGCGGCCGCAT
GATCATCGTCCCCCACCCGAAGAAAGACCTGCCGTTGGGAACGGCGCGGAGCATAGCGAAAATGGCGGGGTGGCTGTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8575.58 Da
• Isoelectric Point: 4.3719

>AT206709 WP_039756568.1 NZ_CP077091:3714094-3714324 [Pseudomonas hamedanensis]
MEQTTIFMSNRSQAVRLPKAVAMPGDVKRVEVIAIGRTRVITPAGESWDSWFDGDSVSPDFMADREQPTDQERESF

Nucleotide

• Length: 231 bp

>AT206709 NZ_CP102664:c129616-129221 [Sphingobium sp. JS3065]
ATGATGAAATATTTCTACGCCATCGTGCACAAGGATCCTGACAGCGCCTATGGCGTCACCTTCCCCGATCTTCCGGGCTG
CTTTTCTGCGGCCGACCGGGAGGAGGAAGTGCTGCCCAACGCCGTCGAGGCGCTCGACCTGTGGTTTGAGGATGCCGACG
ATGTGCAGCCCCGGCCCATCGATCAGATCCGCGCGGCAGTCGCGGACGAGCTGGCGCAGGGCGCTTATCTGTTGGCGGTC
CCCCGGATCAGCGCAGCCGGGAAGATAACCCGCGTCAACCTCTCGATGGATCGCGGCATGCTGGACGCGATCGATGACGC
CGCCGCCCTGCGGAAGCTCACCCGCAGCGCCTTCATGATCGAAGCGGCGCGGAACGAGATCGAGGGGCGGCATTGA