Detailed information of TA system TA206697

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 514162..514805
• Replicon: chromosome
• Accession: NZ_CP077089
• Organism: Pseudomonas tensinigenes strain ZA 5.3

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A4R7VCK5
• Locus tag: HU718_RS02240
• Protein ID: WP_026000492.1
• Coordinates: 514162..514344 (+)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: HU718_RS02245
• Protein ID: WP_150708876.1
• Coordinates: 514404..514805 (+)
• Length: 134 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HU718_RS02225 (HU718_002225)	510277..511203	+	927	WP_095122234.1	serine acetyltransferase	-
HU718_RS02230 (HU718_002230)	511604..513565	+	1962	WP_186616653.1	choline transporter BetT	-
HU718_RS02235 (HU718_002235)	513590..513850	-	261	WP_095122232.1	hypothetical protein	-
HU718_RS02240 (HU718_002240)	514162..514344	+	183	WP_026000492.1	type II toxin-antitoxin system HicA family toxin	Toxin
HU718_RS02245 (HU718_002245)	514404..514805	+	402	WP_150708876.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
HU718_RS02250 (HU718_002250)	514811..515848	-	1038	WP_186616641.1	LacI family DNA-binding transcriptional regulator	-
HU718_RS02255 (HU718_002255)	516054..518279	+	2226	WP_150708874.1	TonB-dependent receptor	-
HU718_RS02260 (HU718_002260)	518281..519600	+	1320	WP_186616640.1	LLM class flavin-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-127)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6903.02 Da
• Isoelectric Point: 10.5417

>T206697 WP_026000492.1 NZ_CP077089:514162-514344 [Pseudomonas tensinigenes]
MRSREMIRMIEDDGWYLVAVKGSHHQYKHPHKPGRVTIKHPDSDLPKGTINSILKQAGLK

Nucleotide

• Length: 183 bp

>T206697 NZ_CP102604:392398-392574 [Pantoea sp. SOD02]
TTGAGCAGCAGGGAGCTGATCAGGATGTTGTTGGAGCGAGGGTGGGTGTTAGATCGTGTTAAGGGAAGCCATCATATATT
CGTACGAATGGACAAGCCTTACCATATTTCGCTCCCTCATCCTGAGAAAGACCTGGCAACTGGAACAGTACGTAAGCTAC
TTAAGCTAATGAACTAA

Antitoxin

Protein

• Length: 134 a.a.
• Molecular weight: 14696.51 Da
• Isoelectric Point: 4.4631

>AT206697 WP_150708876.1 NZ_CP077089:514404-514805 [Pseudomonas tensinigenes]
MKFPVVLHKDADSDYGVIIPDVPGCFSAGTTVAEAFENTQEALALHYEGLVADSAPLPQVRDIDVHLDNPDYAGGIWGVV
DFDITPYFGKSVRFNATLPEQLLERIDQTVRRDQRYRSRSGFLAAAALRELSV

Nucleotide

• Length: 402 bp

>AT206697 NZ_CP102604:392692-393096 [Pantoea sp. SOD02]
ATGAGATTCCCCGTTTATCTTCATAAGACAGAAAGCAACACCTATTCTGGCTTTGTACCGGATGTTGTTGGATGTTTTTT
TGCTGGCGAGACAGTGGATGATGCATTAACTGATGCTTCTGCTGCGATCGATGCGCATGTAGAGTCGTCGACTGATGCGG
GTTACGAGGTGCCGGTGGCAACGAGCATTGAAACGCATATTGAGGATGAAAATTGTCAGGGTGGTTATTGGGCCTTTGTC
GATATCGATCTCAGCCGCTATGAAGGCAAAGCGGTAAAACTCAACATCACCTTGCCACAGAATCTATTAACTAAAATCGA
TAGCTATGTCGATCATCATCGTGAATATGGTTCCAGAAGTGGATTTTTGGCCGCGCTAGCGCGCAAAGAGTTAGCAAACG
CCTGA

Structures

Toxin

• AlphaFold DB: A0A4R7VCK5