Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 2083894..2084551 Replicon chromosome
Accession NZ_CP077085
Organism Pseudomonas shahriarae strain SWRI52

Toxin (Protein)


Gene name hicA Uniprot ID -
Locus tag HU773_RS09530 Protein ID WP_169989398.1
Coordinates 2084366..2084551 (-) Length 62 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag HU773_RS09525 Protein ID WP_169989399.1
Coordinates 2083894..2084316 (-) Length 141 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HU773_RS09485 (HU773_009485) 2078921..2079589 + 669 WP_186625546.1 phage antirepressor KilAC domain-containing protein -
HU773_RS09490 (HU773_009490) 2079589..2080341 + 753 WP_186625534.1 DnaT-like ssDNA-binding domain-containing protein -
HU773_RS09495 (HU773_009495) 2080341..2081027 + 687 WP_186625535.1 replication protein P -
HU773_RS09500 (HU773_009500) 2081226..2081534 + 309 WP_034127829.1 DUF1364 domain-containing protein -
HU773_RS09505 (HU773_009505) 2081531..2082826 + 1296 WP_186625536.1 site-specific integrase -
HU773_RS09510 (HU773_009510) 2082829..2083032 + 204 WP_200660196.1 hypothetical protein -
HU773_RS09515 (HU773_009515) 2083029..2083349 + 321 WP_169989400.1 hypothetical protein -
HU773_RS09520 (HU773_009520) 2083353..2083706 + 354 WP_186625537.1 antiterminator Q family protein -
HU773_RS09525 (HU773_009525) 2083894..2084316 - 423 WP_169989399.1 helix-turn-helix domain-containing protein Antitoxin
HU773_RS09530 (HU773_009530) 2084366..2084551 - 186 WP_169989398.1 type II toxin-antitoxin system HicA family toxin Toxin
HU773_RS09535 (HU773_009535) 2084675..2085007 + 333 WP_169989397.1 phage holin, lambda family -
HU773_RS09540 (HU773_009540) 2085071..2085250 + 180 WP_186625538.1 hypothetical protein -
HU773_RS09545 (HU773_009545) 2085241..2085615 + 375 WP_169989395.1 HNH endonuclease signature motif containing protein -
HU773_RS09550 (HU773_009550) 2085775..2086260 + 486 WP_186625539.1 phage terminase small subunit P27 family -
HU773_RS09555 (HU773_009555) 2086261..2087982 + 1722 WP_186625540.1 terminase large subunit -
HU773_RS09560 (HU773_009560) 2087982..2089253 + 1272 WP_186625541.1 phage portal protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 2065082..2106505 41423


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-59)

Antitoxin

Cro/C1-type helix-turn-helix domain

(82-112)


Sequences

Toxin        


Download         Length: 62 a.a.        Molecular weight: 6878.05 Da        Isoelectric Point: 10.6590

>T206688 WP_169989398.1 NZ_CP077085:c2084551-2084366 [Pseudomonas shahriarae]
MKCNEFKRWLLAQGVEVSKTAKGSHFKIYFNGKQTTLPNHGAKEMAEGTRKAIIKQLGLKD

Download         Length: 186 bp

>T206688 NZ_CP102594:3620150-3620407 [Xanthomonas sp. CFBP 8445]
GTGACGCTGCAGTTCTCGGACAACGCCTGGGAGGATTATCTCTACTGGCAACAGGCCGACAAAAAGATGCTCAAGCGCAT
CAACGACCTGATCAAGGCCATTCAACGCGATCCCTTCCAGGGCATCGGCAAACCCGAACCGCTACGCCATGCACTTGCAG
GCTACTGGTCGCGACGCATCAACGACGAACACCGGATCATCTATAAGGTCGAGCATGGCGTCCTGCTGATTGCACAGGTC
CGATATCACTACGTGTAA

Antitoxin


Download         Length: 141 a.a.        Molecular weight: 15283.42 Da        Isoelectric Point: 4.9914

>AT206688 WP_169989399.1 NZ_CP077085:c2084316-2083894 [Pseudomonas shahriarae]
MYDYAISVTEEAGSFWSSCDDIPEAHSAGDTLEELLTHAAEGLEVALSIYVDQLRHIPKASPPKDGQHVVRLSALVVAKV
ELWNAMRDKGMRKADLCRLLGAAQTKVDRLVDFEHSSKIEQVESALAALGKRLVVSVEPA

Download         Length: 423 bp

>AT206688 NZ_CP102594:3619902-3620153 [Xanthomonas sp. CFBP 8445]
ATGGACACCATCACCTACAGCGCCGCACGTGCCGCTCTTGCGGACACCATGGACCGCGTGGTCAACAACCACGAGCCGGT
GATCATCACGCGCAGCCGCGAGCAAGCCGTGGTCATGCTGTCGCTGGAGGACTACAAGGCGATGGAGGAAACCGCCTATC
TGCTGCGCAGTCCGAAGAACGCGCAGCGCCTGCTGGAATCCATCGCCCAACTCGAATCCGGTCGCGGCAAGGCACGGGAA
CTGAGCGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References