Detailed information of TA system TA206670

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 5596603..5597645
• Replicon: chromosome
• Accession: NZ_CP077082
• Organism: Pseudomonas sp. OE 28.3

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: HU759_RS25120
• Protein ID: WP_099549466.1
• Coordinates: 5596603..5597178 (-)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: A0A554T9A9
• Locus tag: HU759_RS25125
• Protein ID: WP_017135424.1
• Coordinates: 5597175..5597645 (-)
• Length: 157 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HU759_RS25100 (HU759_025100)	5593488..5593817	+	330	WP_099489620.1	hypothetical protein	-
HU759_RS25105 (HU759_025105)	5594017..5594667	-	651	WP_099549468.1	glutathione S-transferase	-
HU759_RS25110 (HU759_025110)	5594715..5595350	-	636	WP_186618493.1	LysE family translocator	-
HU759_RS25115 (HU759_025115)	5595886..5596194	-	309	WP_017135426.1	DUF3077 domain-containing protein	-
HU759_RS25120 (HU759_025120)	5596603..5597178	-	576	WP_099549466.1	PIN domain-containing protein	Toxin
HU759_RS25125 (HU759_025125)	5597175..5597645	-	471	WP_017135424.1	helix-turn-helix domain-containing protein	Antitoxin
HU759_RS25130 (HU759_025130)	5597837..5598991	+	1155	WP_170040551.1	colicin E3/pyocin S6 family cytotoxin	-
HU759_RS25135 (HU759_025135)	5598998..5599255	+	258	WP_169988399.1	colicin E3-like toxin immunity protein	-
HU759_RS25140 (HU759_025140)	5599453..5600826	-	1374	WP_186618081.1	ATP-binding protein	-
HU759_RS25145 (HU759_025145)	5600823..5601485	-	663	WP_017135419.1	response regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-115)

Antitoxin

• Helix-turn-helix domain, group 17 (84-132)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21464.81 Da
• Isoelectric Point: 5.2675

>T206670 WP_099549466.1 NZ_CP077082:c5597178-5596603 [Pseudomonas sp. OE 28.3]
MRCSPFTAVYDACVLYPAALRDLLMWLGLSGLYRARWTSQIHEEWKRNVLKNRPDLTAEQVNCTSALMDKAIPDALVTGY
EDLCSSLDLPDPDDRHVLAAAIRSKAEVIVTFNLKDFPAHILAAYDIEPMHPDDFISDLWDLDQAAVLGAAQKQRRTLKR
PPMEAGSYLEMLMRQGLVQTSKVLGPYGVML

Nucleotide

• Length: 576 bp

>T206670 NZ_CP102553:36386-36802 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGCCACGCCATCGCGGCCGGCGCCATCCTGGTGACGAATAATACAAGAGAGTTTGCGCGGGTCCCGGGCCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin

Protein

• Length: 157 a.a.
• Molecular weight: 17384.04 Da
• Isoelectric Point: 5.6722

>AT206670 WP_017135424.1 NZ_CP077082:c5597645-5597175 [Pseudomonas sp. OE 28.3]
MITNQLPKTILPLEKEVEAAVQGQRELASLLSTKFETQRIDIFDKEDKPHTLVLPTSALRLLVDILGELAIGNAVKVVPV
HAELTSQEAADLLNVSRPHLVKMLEEGAIPFTKTGRHRRIRFSDLMAFKQRRDEESQEAMEALAQQAQALGMGYDG

Nucleotide

• Length: 471 bp

>AT206670 NZ_CP102553:36159-36389 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGAGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Structures

Antitoxin

• AlphaFold DB: A0A554T9A9