Detailed information of TA system TA206669

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 5251918..5252546
• Replicon: chromosome
• Accession: NZ_CP077082
• Organism: Pseudomonas sp. OE 28.3

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: HU759_RS23490
• Protein ID: WP_186618120.1
• Coordinates: 5251918..5252316 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A7Y8FN46
• Locus tag: HU759_RS23495
• Protein ID: WP_017134652.1
• Coordinates: 5252316..5252546 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HU759_RS23465 (HU759_023465)	5247271..5247540	+	270	WP_017138434.1	DUF2790 domain-containing protein	-
HU759_RS23470 (HU759_023470)	5248117..5249367	+	1251	WP_017134657.1	ribonucleotide-diphosphate reductase subunit beta	-
HU759_RS23475 (HU759_023475)	5249726..5250262	+	537	WP_186618151.1	Bro-N domain-containing protein	-
HU759_RS23480 (HU759_023480)	5250362..5251075	+	714	WP_186618118.1	HNH endonuclease signature motif containing protein	-
HU759_RS23485 (HU759_023485)	5251147..5251791	+	645	WP_099550488.1	HAD-IB family hydrolase	-
HU759_RS23490 (HU759_023490)	5251918..5252316	-	399	WP_186618120.1	tRNA(fMet)-specific endonuclease VapC	Toxin
HU759_RS23495 (HU759_023495)	5252316..5252546	-	231	WP_017134652.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
HU759_RS23500 (HU759_023500)	5252832..5253791	-	960	WP_186618122.1	LysR family transcriptional regulator	-
HU759_RS23505 (HU759_023505)	5253985..5255412	+	1428	WP_186618123.1	argininosuccinate lyase	-
HU759_RS23510 (HU759_023510)	5255571..5256449	+	879	WP_017134649.1	amino acid ABC transporter permease	-
HU759_RS23515 (HU759_023515)	5256474..5257238	+	765	WP_017134648.1	amino acid ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-124)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14946.19 Da
• Isoelectric Point: 8.0769

>T206669 WP_186618120.1 NZ_CP077082:c5252316-5251918 [Pseudomonas sp. OE 28.3]
MLKFMLDTNICIFTIKNKPQQVRETFKRHHGQLGISTVTLMELIYVAEKSANPERNLADVEGFAARLEVLKYDQDAAIHT
GQLRAELARAGTPIGPYDQMIAGHARSQGLILVTNNRREFDRVPGLRVEDWV

Nucleotide

• Length: 399 bp

>T206669 NZ_CP102552:c5201669-5201286 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8514.40 Da
• Isoelectric Point: 4.2683

>AT206669 WP_017134652.1 NZ_CP077082:c5252546-5252316 [Pseudomonas sp. OE 28.3]
MDQGSVFQSNRSQAVRLPKAAAMPDDVKRVDVISIGRARIITPAGESWDSWFDGEGVTPDFMTDREQPAEQERESF

Nucleotide

• Length: 231 bp

>AT206669 NZ_CP102552:c5201911-5201669 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Antitoxin

• AlphaFold DB: A0A7Y8FN46