Detailed information of TA system TA206659

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pmenTA/darT(toxin)
• Location: 5515095..5516821
• Replicon: chromosome
• Accession: NZ_CP077081
• Organism: Pseudomonas atacamensis strain SWRI76

Toxin (Protein)

• Gene name: pmenT
• Uniprot ID: -
• Locus tag: KSS92_RS24385
• Protein ID: WP_217878884.1
• Coordinates: 5515095..5515739 (+)
• Length: 215 a.a.

Antitoxin (Protein)

• Gene name: pmenA
• Uniprot ID: -
• Locus tag: KSS92_RS24390
• Protein ID: WP_125918227.1
• Coordinates: 5515736..5516821 (+)
• Length: 362 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KSS92_RS24355 (KSS92_24355)	5510427..5511077	+	651	WP_016773001.1	DedA family protein	-
KSS92_RS24360 (KSS92_24360)	5511082..5511894	+	813	WP_016773000.1	zinc-dependent peptidase	-
KSS92_RS24365 (KSS92_24365)	5512007..5512534	+	528	WP_003205933.1	inorganic diphosphatase	-
KSS92_RS24370 (KSS92_24370)	5512752..5513498	+	747	WP_073475694.1	S24 family peptidase	-
KSS92_RS24375 (KSS92_24375)	5513907..5514341	+	435	WP_225914394.1	hypothetical protein	-
KSS92_RS24380 (KSS92_24380)	5514519..5515094	+	576	WP_024014471.1	DarT ssDNA thymidine ADP-ribosyltransferase family protein	-
KSS92_RS24385 (KSS92_24385)	5515095..5515739	+	645	WP_217878884.1	DUF4433 domain-containing protein	Toxin
KSS92_RS24390 (KSS92_24390)	5515736..5516821	+	1086	WP_125918227.1	macro domain-containing protein	Antitoxin
KSS92_RS24395 (KSS92_24395)	5516867..5517679	-	813	WP_110602926.1	DUF2135 domain-containing protein	-
KSS92_RS24400 (KSS92_24400)	5517683..5519302	-	1620	WP_217878885.1	DUF2300 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ssDNA thymidine ADP-ribosyltransferase, DarT (14-213)

Antitoxin

• Macro domain (18-130)

Sequences

Toxin

Protein

• Length: 215 a.a.
• Molecular weight: 24842.41 Da
• Isoelectric Point: 6.9721

>T206659 WP_217878884.1 NZ_CP077081:5515095-5515739 [Pseudomonas atacamensis]
MNYTSLNPEKALIWRIVHRDNLPWILDNGLHCPTSEAQAPEYVNIGNVDLIDKRRSRQVPIAPEGVLADYVPFYFTPFSV
MMQNIHSGWSVQQRSNDEIVILVSSLYRVKELGLPFVFTNAHAYPSWTNYYSDLANLDQIDWSILQRRDFKRDPDDPRKM
ERYQAEALIHHHLPITGLLGIMCHTDAMKVRIEADVAARGLTLPVHARPKWYFQ

Nucleotide

• Length: 645 bp

>T206659 NZ_CP102552:328245-328613 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCAGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACTCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCGGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCGGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGCTTGCGCGGATGA

Antitoxin

Protein

• Length: 362 a.a.
• Molecular weight: 40397.34 Da
• Isoelectric Point: 6.6149

>AT206659 WP_125918227.1 NZ_CP077081:5515736-5516821 [Pseudomonas atacamensis]
MIRFTQGNLLEAKTEALVNTVNTVGVMGKGIALMFKERFAQNYRVYAAACKAGEVETGKMHVTATNELDGPRWIVNFPTK
RHWRSPSQMAWITEGLHDLRRFLLENDVKSVAVPPLGAGNGGLKWPDVREQIVEVLGDLDADVLVFEPSNQYLNVAKRAG
VEKLTPARALIAELVRRYWVLGMECSLLEIQKLAWFLERAIEQLPDTENPLNLKFVAHKYGPYANRLEHLLDNLDGSYLH
CDKRISDAGINDVIWFDQDRKAFLQTYLKTEAKAYSQALERTAQLIDGFESPFGMELLATVDWLLSQEGVSATVPALREA
LKHWEGGAGAAARKSKLFDDQALDIALKRLTSSTFRPGLAA

Nucleotide

• Length: 1086 bp

>AT206659 NZ_CP102552:328027-328248 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGACGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA