Detailed information of TA system TA206656

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 1171181..1171770
• Replicon: chromosome
• Accession: NZ_CP077081
• Organism: Pseudomonas atacamensis strain SWRI76

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: KSS92_RS05075
• Protein ID: WP_217879323.1
• Coordinates: 1171519..1171770 (-)
• Length: 84 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: V8R847
• Locus tag: KSS92_RS05070
• Protein ID: WP_016771749.1
• Coordinates: 1171181..1171522 (-)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KSS92_RS05045 (KSS92_05045)	1166885..1167286	+	402	WP_016771753.1	30S ribosome-binding factor RbfA	-
KSS92_RS05050 (KSS92_05050)	1167291..1168208	+	918	WP_024011571.1	tRNA pseudouridine(55) synthase TruB	-
KSS92_RS05055 (KSS92_05055)	1168352..1168621	+	270	WP_016771751.1	30S ribosomal protein S15	-
KSS92_RS05060 (KSS92_05060)	1168826..1170931	+	2106	WP_024011572.1	polyribonucleotide nucleotidyltransferase	-
KSS92_RS05070 (KSS92_05070)	1171181..1171522	-	342	WP_016771749.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
KSS92_RS05075 (KSS92_05075)	1171519..1171770	-	252	WP_217879323.1	type II toxin-antitoxin system HicA family toxin	Toxin
KSS92_RS05080 (KSS92_05080)	1172126..1172425	+	300	WP_217879324.1	DUF6388 family protein	-
KSS92_RS05085 (KSS92_05085)	1172474..1173322	-	849	WP_016771747.1	carboxylating nicotinate-nucleotide diphosphorylase	-
KSS92_RS05090 (KSS92_05090)	1173590..1175767	+	2178	WP_136491930.1	DUF1631 domain-containing protein	-
KSS92_RS05095 (KSS92_05095)	1175832..1176392	+	561	WP_136491931.1	1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (20-76)

Antitoxin

• Uncharacterised protein family HicB (55-105)

Sequences

Toxin

Protein

• Length: 84 a.a.
• Molecular weight: 9183.59 Da
• Isoelectric Point: 10.3167

>T206656 WP_217879323.1 NZ_CP077081:c1171770-1171519 [Pseudomonas atacamensis]
MSKTEKLLAKLINGHSAFTWSELVTLLGRLGYSQLEGSGSRIKFDNGDPNALINLHKPHPGNEIKTYARRQIIEQLKTGG
LIP

Nucleotide

• Length: 252 bp

>T206656 NZ_CP102548:c97011-96706 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12594.21 Da
• Isoelectric Point: 5.9655

>AT206656 WP_016771749.1 NZ_CP077081:c1171522-1171181 [Pseudomonas atacamensis]
MSSQLHYKGYVGSIEASTEDKCLFGKLLFIRALVNYEGQTVAELEAAFREAVDDYLQTCEQLGQKPEIPCKGSFNVRIGH
DLHLAAALAATRQNVSLNDLTRQALNEYLQHHA

Nucleotide

• Length: 342 bp

>AT206656 NZ_CP102548:c97231-97013 [Klebsiella pneumoniae]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Structures

Antitoxin

• AlphaFold DB: V8R847