Detailed information of TA system TA206653

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 4976425..4977051
• Replicon: chromosome
• Accession: NZ_CP077080
• Organism: Pseudomonas alvandae strain SWRI17

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: KSS97_RS22000
• Protein ID: WP_217860154.1
• Coordinates: 4976425..4976640 (+)
• Length: 72 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: KSS97_RS22005
• Protein ID: WP_030138469.1
• Coordinates: 4976641..4977051 (+)
• Length: 137 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KSS97_RS21965 (KSS97_21965)	4972379..4973263	+	885	WP_030138315.1	alpha/beta hydrolase	-
KSS97_RS21975 (KSS97_21975)	4973605..4974165	+	561	WP_217860150.1	hypothetical protein	-
KSS97_RS21980 (KSS97_21980)	4974208..4974393	-	186	WP_217860151.1	hypothetical protein	-
KSS97_RS21985 (KSS97_21985)	4974512..4974706	-	195	WP_217860152.1	hypothetical protein	-
KSS97_RS21990 (KSS97_21990)	4974909..4975385	-	477	WP_198797118.1	Lrp/AsnC family transcriptional regulator	-
KSS97_RS21995 (KSS97_21995)	4975530..4976159	+	630	WP_217860153.1	LysE family translocator	-
KSS97_RS22000 (KSS97_22000)	4976425..4976640	+	216	WP_217860154.1	type II toxin-antitoxin system HicA family toxin	Toxin
KSS97_RS22005 (KSS97_22005)	4976641..4977051	+	411	WP_030138469.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
KSS97_RS22010 (KSS97_22010)	4977175..4977321	-	147	WP_217860155.1	hypothetical protein	-
KSS97_RS22015 (KSS97_22015)	4977498..4977902	+	405	WP_030138468.1	low affinity iron permease family protein	-
KSS97_RS22020 (KSS97_22020)	4977948..4978856	-	909	WP_030138467.1	SDR family oxidoreductase	-
KSS97_RS22025 (KSS97_22025)	4978934..4979437	-	504	WP_030138466.1	DUF4142 domain-containing protein	-
KSS97_RS22030 (KSS97_22030)	4979787..4981136	-	1350	WP_217860156.1	GGDEF domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (11-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-128)

Sequences

Toxin

Protein

• Length: 72 a.a.
• Molecular weight: 7983.28 Da
• Isoelectric Point: 10.2823

>T206653 WP_217860154.1 NZ_CP077080:4976425-4976640 [Pseudomonas alvandae]
VNSRFLISQTVADGWYLVRIRGSHHHFKHPTKPGLVTVPHPKKDLLKKTAISILQQALLQTASCPVFPEDD

Nucleotide

• Length: 216 bp

>T206653 NZ_CP102546:4421744-4422154 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAAATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin

Protein

• Length: 137 a.a.
• Molecular weight: 14770.78 Da
• Isoelectric Point: 4.4818

>AT206653 WP_030138469.1 NZ_CP077080:4976641-4977051 [Pseudomonas alvandae]
MLYPIAISMGDDKHAWGVEVPDIPGCFSAGDDLDEAMAMAREAIEGHFEILAEDGSPIPSANTVTLHAADPRYAGCTWAL
VDIDVTKYLGKAQKLNITLPGYLLNRIDEYVLNHPEEKSRSGFLASAALKVLQQGR

Nucleotide

• Length: 411 bp

>AT206653 NZ_CP102546:4421497-4421763 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA