Detailed information of TA system TA206643

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 321281..321797
• Replicon: chromosome
• Accession: NZ_CP077078
• Organism: Pseudomonas azerbaijanoriens strain SWRI123

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: KSS91_RS01430
• Protein ID: WP_217836836.1
• Coordinates: 321516..321797 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: J2QY16
• Locus tag: KSS91_RS01425
• Protein ID: WP_007975643.1
• Coordinates: 321281..321526 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KSS91_RS01395 (KSS91_01395)	316745..318259	+	1515	WP_217836832.1	TolC family protein	-
KSS91_RS01400 (KSS91_01400)	318243..318848	+	606	WP_217836833.1	adenylyl-sulfate kinase	-
KSS91_RS01405 (KSS91_01405)	318860..319618	-	759	WP_217836834.1	slipin family protein	-
KSS91_RS01410 (KSS91_01410)	319621..320154	-	534	WP_015092957.1	NfeD family protein	-
KSS91_RS01415 (KSS91_01415)	320354..320815	+	462	WP_217836835.1	YbaK/EbsC family protein	-
KSS91_RS01420 (KSS91_01420)	320953..321198	+	246	WP_015092958.1	DUF2789 domain-containing protein	-
KSS91_RS01425 (KSS91_01425)	321281..321526	+	246	WP_007975643.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
KSS91_RS01430 (KSS91_01430)	321516..321797	+	282	WP_217836836.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KSS91_RS01435 (KSS91_01435)	321930..322358	+	429	WP_172435674.1	winged helix DNA-binding protein	-
KSS91_RS01440 (KSS91_01440)	322355..324427	+	2073	WP_217836837.1	FUSC family protein	-
KSS91_RS01445 (KSS91_01445)	324424..324633	+	210	WP_007975639.1	DUF1656 domain-containing protein	-
KSS91_RS01450 (KSS91_01450)	324630..325517	+	888	WP_217836838.1	HlyD family secretion protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (9-71)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11013.79 Da
• Isoelectric Point: 10.2255

>T206643 WP_217836836.1 NZ_CP077078:321516-321797 [Pseudomonas azerbaijanoriens]
MTYKLEFLPSARKEWDKLGHTVREQFKKKLSERLEVPRIQSDALHGMPDCYKIKLRSSGYRLVYEVIDERIVVSVVAVGK
RERSEVYERASKR

Nucleotide

• Length: 282 bp

>T206643 NZ_CP102542:2718744-2719004 [Lacticaseibacillus rhamnosus]
ATGATTAAAACCTGGACCGATGATGCTTGGGCGGACTACATGTATTGGCATGATCAAAACGACAAGCGGACAATCAAACG
AATTAATCAACTCATTCAAGCCATTGACCGTGACCCTTATAAAGGCATCGGAAAACCTGAGCCACTTAGATATGCGCTAA
CCGGAAAATGGTCACGTCGGATTGATCAGGAAAATCGCATCATCTACAGCATTGAAAAGAACCACATTAATATTTTCGCC
TGCCGCACTCACTACAGTTAA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8896.26 Da
• Isoelectric Point: 4.7124

>AT206643 WP_007975643.1 NZ_CP077078:321281-321526 [Pseudomonas azerbaijanoriens]
MQNVLADMAVSVSELKKNPSAVLNGAHGGPVAVLNHNRVMGYMVPADVFEAMMERLDDLELAELVRSRRHETPIPVTLDD
L

Nucleotide

• Length: 246 bp

>AT206643 NZ_CP102542:2718482-2718751 [Lacticaseibacillus rhamnosus]
ATGGAAGCAACGAATTATAGTGATTTCCGCCGCAACCTTAAGCATTATATGAGTCAAGTCAACGAAGACGCCGAACCGCT
ACTGGTTACCGCTAAAGATGATGATGACAATGTGGTGGTTATGAGCAAGCACGATTTTGACGCCATCGAAGAAACCCTGT
ATTTACTCAGCAATCCCAAGCTGATGGCCAAAATCAAACGTGGTGATGCCCAAATTGCCGCTGGAAAGGCTAAACAGCAC
GAGTTGTTAACGGACTTCGATCATGATTAA

Structures

Antitoxin

• AlphaFold DB: J2QY16