Detailed information of TA system TA206631

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 5848667..5849298
• Replicon: chromosome
• Accession: NZ_CP077074
• Organism: Pseudomonas sessilinigenes strain CMR12a

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: KSS89_RS26340
• Protein ID: WP_124347476.1
• Coordinates: 5848667..5849065 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A6G7A756
• Locus tag: KSS89_RS26345
• Protein ID: WP_068588739.1
• Coordinates: 5849065..5849298 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KSS89_RS26315 (KSS89_26315)	5843712..5844077	+	366	WP_031320450.1	hypothetical protein	-
KSS89_RS26320 (KSS89_26320)	5844083..5844685	-	603	WP_124347479.1	hypothetical protein	-
KSS89_RS26325 (KSS89_26325)	5844846..5846891	-	2046	WP_124347478.1	c-type cytochrome	-
KSS89_RS26330 (KSS89_26330)	5847107..5847772	-	666	WP_068588881.1	metalloregulator ArsR/SmtB family transcription factor	-
KSS89_RS26335 (KSS89_26335)	5847881..5848384	+	504	WP_068588884.1	DUF2938 domain-containing protein	-
KSS89_RS26340 (KSS89_26340)	5848667..5849065	-	399	WP_124347476.1	tRNA(fMet)-specific endonuclease VapC	Toxin
KSS89_RS26345 (KSS89_26345)	5849065..5849298	-	234	WP_068588739.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
KSS89_RS26350 (KSS89_26350)	5849475..5850056	+	582	WP_124348383.1	DedA family protein	-
KSS89_RS26355 (KSS89_26355)	5850177..5851424	+	1248	WP_124347475.1	aspartate aminotransferase family protein	-
KSS89_RS26360 (KSS89_26360)	5851450..5852034	-	585	WP_068588731.1	hypothetical protein	-
KSS89_RS26365 (KSS89_26365)	5852039..5852482	-	444	WP_068588728.1	DUF3592 domain-containing protein	-
KSS89_RS26370 (KSS89_26370)	5852630..5853460	-	831	WP_124347474.1	amidohydrolase family protein	-
KSS89_RS26375 (KSS89_26375)	5853621..5854016	+	396	WP_124347473.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-124)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14924.13 Da
• Isoelectric Point: 7.8510

>T206631 WP_124347476.1 NZ_CP077074:c5849065-5848667 [Pseudomonas sessilinigenes]
MFKYMLDTNICIFTIKNKPQQVREAFSRNQGRLCISSITLMELIYGAEKSAVPEKNLGRVESFVARLQVLDYDDNAAIHS
GQLQAELAQKGNRIGPYDQLIAGHARSLGLTLVTNNLREFERVPGLRVEDWV

Nucleotide

• Length: 399 bp

>T206631 NZ_CP102536:c1044077-1043721 [Bifidobacterium breve]
ATGATGAAGACTGATCCGCACCAGTTCGAGATATGGTGGGTGCCGTTCACTTTTCCCGACAAGCCAGGCAAAGCGAAGAA
CCGTCCCAGCGTGATCCTCCAATGGGACGACGGCACCCGCATCGCCTTGGTGACCAAGGTCACCGGCAACACCTGGCGCG
ACGAACCGGGCTATGTGGTGCTGCGTGACTGGCAGGACGCGGGACTGAGCAAGCCCAGCGCCGTGCGATGCTCGCAACTG
CTGCGGCTGCCAAGCAGTCTTTTCCTCGACGACGGCCCGACAGGAAGACTTTCGGCATATGATGCCGGCCGCGTCACCTG
GGCGATCAACGAACTCTATCCGGGCCTACTAGCCTGA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8732.69 Da
• Isoelectric Point: 4.2092

>AT206631 WP_068588739.1 NZ_CP077074:c5849298-5849065 [Pseudomonas sessilinigenes]
METGSVFKSNRSQAIRMPKAVALPDDVTRVDIVAIGRTRIITPAGESWDSWFDEQDSVSADFMTEREQPAEQVREEF

Nucleotide

• Length: 234 bp

>AT206631 NZ_CP102536:c1044360-1044064 [Bifidobacterium breve]
ATGATGGCTATCAAGGAGAAGACCACGATCAGTCTGGATGCGCAGACCAAGCGTGACGGCATCGCCATACTTGACGCCAT
GGGGTTGAACCTGAGCACGTTTGCCGAAATGTCTCTCAGACAATTGGTACGCGACGGGCGTCTGCCATTCACGCCAAGCG
TCCGCCCTTCATTCGAAAAAGACAACGAGGGATATCCCCTGTTCAAAGCGAACATGGATGATCCCCGCATCGTGACGCCC
CAGATACGTGATGGTGCCGTGATCCTGCCGGAGGGTTGGGACGATGATGAAGACTGA

Structures

Antitoxin

• AlphaFold DB: A0A6G7A756