Detailed information of TA system TA206630

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/-
• Location: 3021595..3022817
• Replicon: chromosome
• Accession: NZ_CP077074
• Organism: Pseudomonas sessilinigenes strain CMR12a

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: KSS89_RS14175
• Protein ID: WP_124346631.1
• Coordinates: 3022245..3022817 (+)
• Length: 191 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U6ZMH2
• Locus tag: KSS89_RS14170
• Protein ID: WP_022639877.1
• Coordinates: 3021595..3022152 (+)
• Length: 186 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KSS89_RS14135 (KSS89_14135)	3016708..3017061	+	354	Protein_2794	ATP-binding protein	-
KSS89_RS14140 (KSS89_14140)	3017131..3017742	-	612	WP_068583852.1	AidA/PixA family protein	-
KSS89_RS14145 (KSS89_14145)	3018013..3018432	+	420	WP_124346629.1	helix-turn-helix domain-containing protein	-
KSS89_RS14150 (KSS89_14150)	3018429..3019304	+	876	WP_217884177.1	alpha/beta fold hydrolase	-
KSS89_RS14155 (KSS89_14155)	3019291..3019677	+	387	WP_068583860.1	DUF4180 domain-containing protein	-
KSS89_RS14160 (KSS89_14160)	3019810..3020988	+	1179	WP_124346630.1	PrpF domain-containing protein	-
KSS89_RS14165 (KSS89_14165)	3021094..3021423	-	330	WP_022639876.1	low molecular weight protein tyrosine phosphatase family protein	-
KSS89_RS14170 (KSS89_14170)	3021595..3022152	+	558	WP_022639877.1	helix-turn-helix transcriptional regulator	Antitoxin
KSS89_RS14175 (KSS89_14175)	3022245..3022817	+	573	WP_124346631.1	HD domain-containing protein	Toxin
KSS89_RS14180 (KSS89_14180)	3022851..3023357	+	507	WP_068583868.1	N-acetyltransferase	-
KSS89_RS14185 (KSS89_14185)	3023369..3025711	-	2343	WP_124346632.1	penicillin-binding protein 1A	-
KSS89_RS14190 (KSS89_14190)	3025753..3027675	-	1923	WP_124346633.1	penicillin-binding protein 2	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• 5'-deoxynucleotidase YfbR/HDDC2 (10-187)

Antitoxin

• Cro/C1-type helix-turn-helix domain (15-49)

Sequences

Toxin

Protein

• Length: 191 a.a.
• Molecular weight: 21520.09 Da
• Isoelectric Point: 5.3042

>T206630 WP_124346631.1 NZ_CP077074:3022245-3022817 [Pseudomonas sessilinigenes]
MNDQLLGGRLEFLRQAERLKDVLRSAHTSSGRQESTAEHSWRLCLMALLLEDQLGELDLLQVLKLCIVHDLGEALHGDIP
AVQQAAHPDKGQQEREDLQTLASCLDAPARQRLLALWDEYENASSAEAQAVKALDKLETLVQHNQGRNPADFDYRFNLDY
GRRYTSATPLFRALREQIDHDTRRHIDAAG

Nucleotide

• Length: 573 bp

>T206630 NZ_CP102536:c984727-984404 [Bifidobacterium breve]
ATGAAACGAGGTGAGATTCGGACGTTGCAGGCCGATGGCTACGCCAGCAAGCCACGGCCGGTTGTCATCGTGCAGAGCGA
TGCCGTCGATCGGTTCGATTCCGTCATCACTTGTCTGCTGACCTCATACGATTCCTCCGACATCGACACGCGGGTCCGAT
TGGAACCAAGTCCGGAGAACGGCCTGAACAAAGTGAGTTACGTGATGACGGACAAGATCGTCACCGTCGACCGCAAACTG
CTGGGATACCAAGTCGGCGTGGTTGATGATGCAGCCATGGCGAATATCGGCCGTCAGCTCATGCGGGTTCTAGGTTTGCT
GTGA

Antitoxin

Protein

• Length: 186 a.a.
• Molecular weight: 20413.32 Da
• Isoelectric Point: 7.0310

>AT206630 WP_022639877.1 NZ_CP077074:3021595-3022152 [Pseudomonas sessilinigenes]
MSASLFTSPHLGLALRRWRLLHRVKQQHAAELFGVAQSSISRWENGQQAMEPSERAKLEQLLAANLSAAADQALARLVND
SPRAVHLVCDLTHRLLACSPTRAGEFGVPLSELLGQSLWGFCTEEIVRKERALDDLGWREVLAPPALEFFSGHNDSAIVP
IAASQCRWTRLNLSDGNAVRLVETL

Nucleotide

• Length: 558 bp

>AT206630 NZ_CP102536:c984959-984714 [Bifidobacterium breve]
ATGAGCATGCAGATTGCCACGCGAGTTGATGACGAGCAGGCTGCGTTGTTTAAGGAGACGACCCGACAGCTGGGTACCAC
CCCGGCCGATGCCCTGCGTATGTTCATCAGCGCGTTCAATGATTACCGTGGGTTCCCCTATGAGGTGCGTTTGCCACGAA
ATGACGTGGAGCCGTTTGCGTCCGAGCGGGACGCTACTGAGTATGCCAGTCGTCTCGCCTTGAGGATGTCCGATGAAACG
AGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A6G7A0D6