Detailed information of TA system TA206628

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-RHH
• Location: 1745788..1746301
• Replicon: chromosome
• Accession: NZ_CP077074
• Organism: Pseudomonas sessilinigenes strain CMR12a

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A6G6ZW42
• Locus tag: KSS89_RS08025
• Protein ID: WP_068582176.1
• Coordinates: 1745788..1746072 (-)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A7X1FE57
• Locus tag: KSS89_RS08030
• Protein ID: WP_068582179.1
• Coordinates: 1746062..1746301 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KSS89_RS08000 (KSS89_08000)	1740948..1741835	-	888	WP_124345962.1	dihydrodipicolinate synthase family protein	-
KSS89_RS08005 (KSS89_08005)	1741869..1742651	-	783	WP_124345963.1	aldolase	-
KSS89_RS08010 (KSS89_08010)	1742813..1743355	+	543	WP_124345964.1	XRE family transcriptional regulator	-
KSS89_RS08015 (KSS89_08015)	1743419..1744438	-	1020	WP_068582209.1	DUF4917 family protein	-
KSS89_RS08020 (KSS89_08020)	1744596..1745744	+	1149	WP_124345965.1	L-threonine dehydrogenase	-
KSS89_RS08025 (KSS89_08025)	1745788..1746072	-	285	WP_068582176.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KSS89_RS08030 (KSS89_08030)	1746062..1746301	-	240	WP_068582179.1	ribbon-helix-helix protein, CopG family	Antitoxin
KSS89_RS08035 (KSS89_08035)	1746498..1747238	+	741	WP_124345966.1	hypothetical protein	-
KSS89_RS08040 (KSS89_08040)	1747249..1747974	+	726	WP_124345967.1	hypothetical protein	-
KSS89_RS08045 (KSS89_08045)	1747978..1748421	+	444	WP_068582188.1	fosfomycin resistance glutathione transferase	-
KSS89_RS08050 (KSS89_08050)	1748523..1749161	+	639	WP_068582191.1	LysE family translocator	-
KSS89_RS08055 (KSS89_08055)	1749271..1750620	+	1350	WP_068582196.1	GGDEF domain-containing protein	-
KSS89_RS08060 (KSS89_08060)	1750744..1751268	+	525	WP_124345968.1	DUF4142 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-41)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 11100.77 Da
• Isoelectric Point: 11.5026

>T206628 WP_068582176.1 NZ_CP077074:c1746072-1745788 [Pseudomonas sessilinigenes]
MHIEWLRTALKNLDDEARYIAQDNPEAAREFVQTLLTGIGHLARFPASGREGRLPGTREWVVPGWPYLIPYRVRGSRLQI
LRIFHTRRQPPQRW

Nucleotide

• Length: 285 bp

>T206628 NZ_CP102534:359860-360201 [Enterococcus faecium]
GTGTCAGAAGAAAGAATTTATATTCCTAAAAAAGGTGATATTGTGTGGATTGATTTTGATCCTTCGGTAGGAAAAGAAAT
TCAAAAAAGACGACCAGGACTAGTCGTCTCTCGTTATGAGTTTAATCGTAAAACAATGTTTTCGGTCATATGTCCGATTA
CTTCCACGATAAAGAATTTACCGACACGTTACTCATTACCAGAAGAGCTAGATATAAAAGGACAAGTACTTATTTCTCAA
TTAAAATCACTTGATTTTAAGGAACGAAGACTCAAAAAAATAGAAAACCTGCCATTGCAAGACATGGCTAAAATTGATCA
GATTATACAATATATGTTTTAA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8537.68 Da
• Isoelectric Point: 4.4143

>AT206628 WP_068582179.1 NZ_CP077074:c1746301-1746062 [Pseudomonas sessilinigenes]
MSIMSLRLPDEMAETLALLAKATGRSKSFLAVDALREYLAREAWQIEEIQKALGEADAGDFASAEEVAAITGKWTGNAH

Nucleotide

• Length: 240 bp

>AT206628 NZ_CP102534:359630-359860 [Enterococcus faecium]
ATGCTTGTTACAAAATCTAGATTACAAGGAAGTTCTGTAGTGGTTACTTTACCTTCTGATAACGGAAAGAAACCTTCAGA
AAACCAAGAATATATCGTTGTATATTCTGACGATGGGACAATTACGTTAGTTCCAAAAATTGAAGATCCATTTAGTGGTG
GAGAAGAGGCCGAATATTATGAAAAAGACGAATGGGAAGATTTAACTCCTGAAGGGAGAGAAAGGTTATAG

Structures

Toxin

• AlphaFold DB: A0A6G6ZW42

Antitoxin

• AlphaFold DB: A0A7X1FE57