Detailed information of TA system TA206626

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 923301..923897
• Replicon: chromosome
• Accession: NZ_CP077074
• Organism: Pseudomonas sessilinigenes strain CMR12a

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A6G6ZTX3
• Locus tag: KSS89_RS04130
• Protein ID: WP_068577099.1
• Coordinates: 923595..923897 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: KSS89_RS04125
• Protein ID: WP_124345642.1
• Coordinates: 923301..923591 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KSS89_RS04105 (KSS89_04105)	919569..921701	-	2133	WP_124345640.1	TonB-dependent copper receptor	-
KSS89_RS04110 (KSS89_04110)	921790..922236	-	447	WP_225926554.1	DUF2946 domain-containing protein	-
KSS89_RS04115 (KSS89_04115)	922259..922738	-	480	WP_176719722.1	copper chaperone PCu(A)C	-
KSS89_RS04120 (KSS89_04120)	922803..923201	-	399	WP_124345641.1	DUF2946 domain-containing protein	-
KSS89_RS04125 (KSS89_04125)	923301..923591	-	291	WP_124345642.1	putative addiction module antidote protein	Antitoxin
KSS89_RS04130 (KSS89_04130)	923595..923897	-	303	WP_068577099.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KSS89_RS04135 (KSS89_04135)	924075..924803	-	729	WP_124345643.1	cobalt-precorrin-6A reductase	-
KSS89_RS04140 (KSS89_04140)	924800..925897	-	1098	WP_068577104.1	cobalt-precorrin-5B (C(1))-methyltransferase	-
KSS89_RS04145 (KSS89_04145)	925890..927134	-	1245	WP_068577106.1	bifunctional cobalt-precorrin-7 (C(5))-methyltransferase/cobalt-precorrin-6B (C(15))-methyltransferase	-
KSS89_RS04150 (KSS89_04150)	927183..928586	+	1404	WP_124345644.1	precorrin-3B synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (4-97)

Antitoxin

• Uncharacterized protein HI1420 (3-94)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11600.24 Da
• Isoelectric Point: 10.8593

>T206626 WP_068577099.1 NZ_CP077074:c923897-923595 [Pseudomonas sessilinigenes]
MFVFEQTQEFSHWLTRLKDPQGKTRVLARIRAAQLGRFGDCVSIGGGVHEMRIHRGPGYRVYFSRRGERIYLLLIGGDKS
TQSRDIQRARQLAHDLKNED

Nucleotide

• Length: 303 bp

>T206626 NZ_CP102530:c2679513-2679130 [Lacticaseibacillus paracasei subsp. paracasei]
ATGGATGTGTCCGTAAAACGCGGCGATGTTTTTTTCGCCGATCTGTCTCCGGTTGTCGGCTCCGAACAAGGCGGCAACCG
GCCAGTACTTATTATTCAAAACAACGTTGGCAACCATTACAGTCCCACAGTGATTGTGGCAGCCATTACCTCCAAGATTC
AAAAACCCAAGATGCCGACGCATGTCGGGTTACGGGCCAAGCAAGATGGCGTTGAACGCAACTCGGTCATTTTACTTGAG
CAAATTCGCACGATTGACAAGCAACGGCTACAAGCCCGCGTGACAGCTCTGTCAAGCACCAAAATGGCAGCAGTCGACCG
CGCGTTGGCGATTTCAGTGGGTCTGGTCAGTTTGCCCAAGCCCAAAACATATAATAAGAATTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10389.97 Da
• Isoelectric Point: 6.2732

>AT206626 WP_124345642.1 NZ_CP077074:c923591-923301 [Pseudomonas sessilinigenes]
MRTPVTRFDAADYLKTPQDMVAYLDACFEEDAGDGVLIRAALNDIARARGMTQIARDTGLGRESLYKALGSQGNPEFATI
IKVMKALGLKLQVSPA

Nucleotide

• Length: 291 bp

>AT206626 NZ_CP102530:c2679781-2679533 [Lacticaseibacillus paracasei subsp. paracasei]
ATGAAAAAGCAAACGTCAGTAGCGGTAACGTTTGATGATGCCATTAAGGCGCGGTTGGATGCCTATTGTGAATTGAATGA
CATTGACGCTCAGGCTGTGATTACGGAGGCAGTGGCGACATTTTTGAAGTCACAGGCTCCCGAAATCAACCAATTGATTT
CTGGCTATGTAGCAATGGGCCAGATCAATACAGAGATCAGTCATGCTTTCTCTGCCTGCGAGAGCGAGGTATACGCCCAT
ATTCGATGA

Structures

Toxin

• AlphaFold DB: A0A6G6ZTX3