Detailed information of TA system TA206620

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 1399984..1400618
• Replicon: chromosome
• Accession: NZ_CP077073
• Organism: Pseudomonas muyukensis strain COW39

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: KSS95_RS06280
• Protein ID: WP_217852584.1
• Coordinates: 1399984..1400166 (+)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: KSS95_RS06285
• Protein ID: WP_217852586.1
• Coordinates: 1400211..1400618 (+)
• Length: 136 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KSS95_RS06255 (KSS95_06255)	1395009..1395632	-	624	WP_217852574.1	lysophospholipid acyltransferase family protein	-
KSS95_RS06260 (KSS95_06260)	1395638..1396057	-	420	WP_217852576.1	hypothetical protein	-
KSS95_RS06265 (KSS95_06265)	1396054..1397379	-	1326	WP_217852578.1	phosphatase PAP2/dual specificity phosphatase family protein	-
KSS95_RS06270 (KSS95_06270)	1397379..1399133	-	1755	WP_217852580.1	bifunctional alpha/beta hydrolase/class I SAM-dependent methyltransferase	-
KSS95_RS06275 (KSS95_06275)	1399164..1399769	-	606	WP_217852582.1	CDP-alcohol phosphatidyltransferase family protein	-
KSS95_RS06280 (KSS95_06280)	1399984..1400166	+	183	WP_217852584.1	type II toxin-antitoxin system HicA family toxin	Toxin
KSS95_RS06285 (KSS95_06285)	1400211..1400618	+	408	WP_217852586.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
KSS95_RS06290 (KSS95_06290)	1400648..1401853	-	1206	WP_217852587.1	methylaspartate mutase	-
KSS95_RS06295 (KSS95_06295)	1401911..1402360	-	450	WP_217852589.1	hypothetical protein	-
KSS95_RS06300 (KSS95_06300)	1402357..1403484	-	1128	WP_225935583.1	fatty acid desaturase	-
KSS95_RS06305 (KSS95_06305)	1403737..1404618	+	882	WP_217852593.1	LysR family transcriptional regulator	-
KSS95_RS06310 (KSS95_06310)	1404631..1405617	-	987	WP_217852594.1	quinone oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-127)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6910.04 Da
• Isoelectric Point: 10.7251

>T206620 WP_217852584.1 NZ_CP077073:1399984-1400166 [Pseudomonas muyukensis]
MRSRDVIRLIEADGWYEVEVKGSHHQFRHPLKRGRVTVAHPMSDLPKGTLYSILKQAGLK

Nucleotide

• Length: 183 bp

>T206620 NZ_CP102513:c4128261-4127914 [Streptomyces sp. DSM 40750]
ATGAAGCGCGGTGACATCTACCTGGTCGACTACGAGCCTGCGCGCGGCAGCGAGGCCAGCAAGGCGCGTCCGGCCGTGAT
CGTTTCCAACGACGGTGCCAACGCGGCTGCGGCACGGACCGGACGCGGCGTGATCACATTGGTCCCCCTGACGACCAACA
CCTCCCGCGTCTATCCGTTCCAAGTGCTGCTTCCTGCCCAGGAGTGCGGTCTCCCGAAGGACTCCAAGGTGCAGTGCGAG
CAGGTGCGCGCGCTCGCTCCGGAGAGGCTCCTGCGGCCGATCGGTTCGGTCCCGCGCCAGCGAATGGCTGAGGTCGACGT
GGCCCTACGACGGCATCTGGCCCTCTGA

Antitoxin

Protein

• Length: 136 a.a.
• Molecular weight: 14687.55 Da
• Isoelectric Point: 4.6143

>AT206620 WP_217852586.1 NZ_CP077073:1400211-1400618 [Pseudomonas muyukensis]
MKFAVVLHKDDDSDYGVTVPDVPGCFSAGRSVSHALDNVLEALALHFEGLVADGEPLPQALEVDVHVDNPDYAGGIWAVV
EFDVTPYLGKAVRFNATLPENLLQRIDEKVKRDHRYASRSGFLASAALRELAVAQ

Nucleotide

• Length: 408 bp

>AT206620 NZ_CP102513:c4128478-4128248 [Streptomyces sp. DSM 40750]
ATGAAGATTAGTGTGAGCCTGCCGCAGGAGGACGTTGCCTTCGTTGACGAGTACGCCGAGCGAACAAAGGCCGATTCCCG
GTCAGCCGTGATACACGCAGCGATCGAACTCCTGCGTGAAGCGCAGCTGGAGCAGGAGTACACGGAGGCCTTCGCCGAGT
GGGACGGGAGCGAGGACGCCGCGTTCTGGGACCAGTTCTCGGGGGACGGGATGACCGATGAAGCGCGGTGA