Detailed information of TA system TA206619

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1288343..1288983
• Replicon: chromosome
• Accession: NZ_CP077073
• Organism: Pseudomonas muyukensis strain COW39

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: KSS95_RS05820
• Protein ID: WP_217852446.1
• Coordinates: 1288343..1288753 (-)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q1I4A9
• Locus tag: KSS95_RS05825
• Protein ID: WP_011535889.1
• Coordinates: 1288753..1288983 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KSS95_RS05795 (KSS95_05795)	1285366..1285797	-	432	WP_217852436.1	DUF2946 domain-containing protein	-
KSS95_RS05800 (KSS95_05800)	1285818..1286303	-	486	WP_217852438.1	copper chaperone PCu(A)C	-
KSS95_RS05805 (KSS95_05805)	1286350..1286739	-	390	WP_217852440.1	DUF2946 domain-containing protein	-
KSS95_RS05810 (KSS95_05810)	1286951..1287805	-	855	WP_217852442.1	SPFH domain-containing protein	-
KSS95_RS05815 (KSS95_05815)	1287833..1288270	-	438	WP_217852444.1	NfeD family protein	-
KSS95_RS05820 (KSS95_05820)	1288343..1288753	-	411	WP_217852446.1	tRNA(fMet)-specific endonuclease VapC	Toxin
KSS95_RS05825 (KSS95_05825)	1288753..1288983	-	231	WP_011535889.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
KSS95_RS05830 (KSS95_05830)	1289043..1289783	-	741	WP_217852448.1	cobalt-precorrin-6A reductase	-
KSS95_RS05835 (KSS95_05835)	1289780..1290874	-	1095	WP_217852450.1	cobalt-precorrin-5B (C(1))-methyltransferase	-
KSS95_RS05840 (KSS95_05840)	1290867..1292078	-	1212	WP_217852451.1	precorrin-6y C5,15-methyltransferase (decarboxylating) subunit CbiE	-
KSS95_RS05845 (KSS95_05845)	1292261..1293571	+	1311	WP_217852453.1	precorrin-3B synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-124)

Antitoxin

• SpoVT-AbrB domain (7-53)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 15250.58 Da
• Isoelectric Point: 9.4581

>T206619 WP_217852446.1 NZ_CP077073:c1288753-1288343 [Pseudomonas muyukensis]
MLRYMLDTNICIFTIKNKPQVVRDAFNRYHGQLAISTVTLMELIYGAEKSAAPERNLAIVEGFAARLDVLDYDIQGAAHT
AQLRAELAKAGTPIGPYDRMIAGHARARGLTLVTNNLREFQRVPGLRVEDWVTHTR

Nucleotide

• Length: 411 bp

>T206619 NZ_CP102512:6617871-6618239 [Streptomyces sp. CA-210063]
ATGCACTACCTCACGATGCCCGAGCTGCTGAATCTCGCACAGCGGCTCGGGGCGGACGAGGTGCGCGACTACGGGCTCCT
GGACTCGGCCCTAGCGCGTCCCCAGTCGAGCGTGTTCGGGCAGGACGCGTACCCGGACGTGTGGCAGAAGGCCGCGGCGT
TGATGGAGTCGCTGGCTCGCAACCATGCCCTTGTCGACGGGAACAAGCGCATCGCCTGGTATGCGACCTGGGTGTACCTG
CACATGAACGGGCACCCCCTGGACGTCGACTTCGACGTGGACGAGGCCGAGCACTTCGTGCTGGATGTCTGCCAGGGCGC
ACTGGACGTACCCAAGATCGCTGCTCAACTGCCGCGCTTCGCGCGCTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8443.36 Da
• Isoelectric Point: 4.2821

>AT206619 WP_011535889.1 NZ_CP077073:c1288983-1288753 [Pseudomonas muyukensis]
MEQGAVFKSNRSQAIRMPKALALPEDVTRVDIVAVGRSRIISPAGESWDSWFEGDDVSADFMASREQPADQEREGF

Nucleotide

• Length: 231 bp

>AT206619 NZ_CP102512:6617674-6617871 [Streptomyces sp. CA-210063]
ATGGCTGGACTGAACCTGCGGTTTACGGATGAAGAGCTCGATGCCCTGCGCGAGCGCGCGGCGGCGGAGGGGCGCAGCAT
GCAGGCCTTCGCGCACGACGCGGTGATCGCGGCCATAAACGAGCACTCAAGACTGTTCAACGAGGCGGCCGAGCATGTGC
TGAAGGCCAGCGCCGAGTTGAACCGGAGGCTCGCCTGA

Structures

Antitoxin

• AlphaFold DB: A0A2V4HYG6