Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 4800714..4801489 Replicon chromosome
Accession NZ_CP076863
Organism Klebsiella pneumoniae strain V3

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1W1JAQ8
Locus tag KRP25_RS23385 Protein ID WP_009308645.1
Coordinates 4801004..4801489 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag KRP25_RS23380 Protein ID WP_004150912.1
Coordinates 4800714..4801007 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KRP25_RS23360 (4795922) 4795922..4796524 - 603 WP_217046663.1 short chain dehydrogenase -
KRP25_RS23365 (4796622) 4796622..4797533 + 912 WP_217046664.1 LysR family transcriptional regulator -
KRP25_RS23370 (4797534) 4797534..4798682 - 1149 WP_217046665.1 PLP-dependent aspartate aminotransferase family protein -
KRP25_RS23375 (4798693) 4798693..4800069 - 1377 WP_217046666.1 pyridoxal-phosphate dependent enzyme -
KRP25_RS23380 (4800714) 4800714..4801007 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
KRP25_RS23385 (4801004) 4801004..4801489 + 486 WP_009308645.1 GNAT family N-acetyltransferase Toxin
KRP25_RS23390 (4801808) 4801808..4802119 - 312 WP_225560083.1 hypothetical protein -
KRP25_RS26820 (4802234) 4802234..4802453 - 220 Protein_4577 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17595.60 Da        Isoelectric Point: 8.5144

>T206595 WP_009308645.1 NZ_CP076863:4801004-4801489 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPDPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T206595 NZ_CP102500:c906075-905668 [Citrobacter youngae]
GTGGTCCAGTGGCAATCTGATTTACGCGTTTCATGGCGCGCACAGTGGCTCTCTCTGCTCATTCATGGGCTGGTTGCGGT
ATTCATTTTATTGATGCCCTGGCCATTGAGCTACACCCCATTATGGTTGGTGCTGCTCTCTCTGGTGGTGTTTGATAGCG
TACGCAGTCAGCGTCGCATTAACGCTTGTCAGGGAGAGATTAGACTGTTAATGGACGGTCGCCTGCGCTGGCAGGGGCAA
GAGTGGTCTATCGTTTGTGCACCCTGGATGATTAAAACGGGCATGATGCTGCGTTTGCGTTCAGATGCGGGAAAACGTCA
GCATTTGTGGCTGGCAGCGGACAGCATGGATGACGCTGAGTGGCGGGATTTGCGGCGGTTAATGCTGCAACAGGTAAAGC
AGGGTTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT206595 WP_004150912.1 NZ_CP076863:4800714-4801007 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT206595 NZ_CP102500:c906322-906056 [Citrobacter youngae]
ATGGATATTAACAACAAAGCACGTATTCATTGGGCATGCCGCCGCGGTATGCGTGAACTGGACATCTCTATCATGCCGTT
TTTCGAACATGAGTATGACAACTTAAGTGACGAAGAAAAACGCATCTTTATCCGTCTGCTGGAATGTGATGATCCAGACT
TATTTAACTGGTTGATGAATCATGGGAAACCGGCTGATGAGCAGTTGGAGCAGATGGTGCGACTCATCCAGACACGGAAT
CGGGAACGTGGTCCAGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1W1JAQ8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References