Detailed information of TA system TA206593

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 3971629..3972254
• Replicon: chromosome
• Accession: NZ_CP076863
• Organism: Klebsiella pneumoniae strain V3

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: KRP25_RS19270
• Protein ID: WP_109226863.1
• Coordinates: 3971629..3972012 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: J2DFR0
• Locus tag: KRP25_RS19275
• Protein ID: WP_004150355.1
• Coordinates: 3972012..3972254 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KRP25_RS19255 (3968995)	3968995..3969897	+	903	WP_217046056.1	formate dehydrogenase subunit beta	-
KRP25_RS19260 (3969894)	3969894..3970529	+	636	WP_217046058.1	formate dehydrogenase cytochrome b556 subunit	-
KRP25_RS19265 (3970526)	3970526..3971455	+	930	WP_217046060.1	formate dehydrogenase accessory protein FdhE	-
KRP25_RS19270 (3971629)	3971629..3972012	-	384	WP_109226863.1	type II toxin-antitoxin system VapC family toxin	Toxin
KRP25_RS19275 (3972012)	3972012..3972254	-	243	WP_004150355.1	CopG family transcriptional regulator	Antitoxin
KRP25_RS19280 (3972459)	3972459..3973376	+	918	WP_217046062.1	alpha/beta hydrolase	-
KRP25_RS19285 (3973390)	3973390..3974331	-	942	WP_217046064.1	bifunctional GNAT family N-acetyltransferase/thioesterase	-
KRP25_RS19290 (3974376)	3974376..3974813	-	438	WP_002882809.1	D-aminoacyl-tRNA deacylase	-
KRP25_RS19295 (3974810)	3974810..3975670	-	861	WP_217046066.1	virulence factor BrkB family protein	-
KRP25_RS19300 (3975664)	3975664..3976263	-	600	WP_151360904.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-112)

Antitoxin

• Predicted DNA-binding protein, ribbon-helix-helix domain (16-47)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14405.67 Da
• Isoelectric Point: 8.5338

>T206593 WP_109226863.1 NZ_CP076863:c3972012-3971629 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHREQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Nucleotide

• Length: 384 bp

>T206593 NZ_CP102500:702660-703001 [Citrobacter youngae]
ATGAAACCTCAATCTGCAGCAACTTCGCGGGCGGTAAAACCCTGCCTGTCACCCGTGGCTATCTGGCAAATTCTACTGAC
ACGCCTGCTGGAACAACACTACGGCCTGACCCTGAACGACACGCCGTTCAGGGATGAATCCGTTATACAGGAACATATCG
ATGCAGGTATCACGCTGGCCAATGCCGTTAACTTTCTGGTGGAAAAATACGAGCTTGTTCGTATCGATCGCAGAAGGTTT
AGTTGGCAGGAACAATCACCTTATCTTCGGGCAGCAGATATTCTGCGAGCACGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAACAACGTAGTACGGTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9567.83 Da
• Isoelectric Point: 4.3509

>AT206593 WP_004150355.1 NZ_CP076863:c3972254-3972012 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Nucleotide

• Length: 243 bp

>AT206593 NZ_CP102500:702322-702639 [Citrobacter youngae]
ATGAGCAACATCACTTGGGGCTTGCAGCGCGATATCACGCCGCGCCTGGGAACACGTCTGGTGCAGGAGGGCAACCGGCT
GCACTACCTAGCTGACCGGGCCAGTATCACCGGTAAGTTCAGTGACGCCGAATGCCAGAAGCTGGATGAAACATTCCCGC
ACTTTATCAGCCAGATGGAATCGATGCTGACCTCCGGTGAACTGAATCCCCTACACGTTCATTGCGTCACCCTGTACCAC
ACCGGTTTTACCTGCGAAGCCGACACCCTCGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTAAGCGCTAA

Structures

Antitoxin

• AlphaFold DB: A0A0H3GGU9