Detailed information of TA system TA206591

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/DUF1778(antitoxin)
• Location: 3350757..3351567
• Replicon: chromosome
• Accession: NZ_CP076863
• Organism: Klebsiella pneumoniae strain V3

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: A0A060VJ83
• Locus tag: KRP25_RS16365
• Protein ID: WP_004178461.1
• Coordinates: 3350757..3351290 (-)
• Length: 178 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: -
• Locus tag: KRP25_RS16370
• Protein ID: WP_217045451.1
• Coordinates: 3351301..3351567 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KRP25_RS16360 (3349588)	3349588..3350709	+	1122	WP_217045450.1	cupin domain-containing protein	-
KRP25_RS16365 (3350757)	3350757..3351290	-	534	WP_004178461.1	type II toxin-antitoxin system toxin KacT	Toxin
KRP25_RS16370 (3351301)	3351301..3351567	-	267	WP_217045451.1	DUF1778 domain-containing protein	Antitoxin
KRP25_RS16375 (3351670)	3351670..3353103	-	1434	WP_217045452.1	Cu(+)/Ag(+) sensor histidine kinase	-
KRP25_RS16380 (3353093)	3353093..3353776	-	684	WP_217045453.1	copper/silver response regulator transcription factor	-
KRP25_RS16385 (3353949)	3353949..3355334	+	1386	WP_217049133.1	efflux transporter outer membrane subunit	-
KRP25_RS16390 (3355352)	3355352..3355696	+	345	WP_021462623.1	cation efflux system protein CusF	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (91-151)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 178 a.a.
• Molecular weight: 19810.65 Da
• Isoelectric Point: 5.2614

>T206591 WP_004178461.1 NZ_CP076863:c3351290-3350757 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Nucleotide

• Length: 534 bp

>T206591 NZ_CP102492:c59513-59031 [Klebsiella pneumoniae]
GTGGGATGTATAACTGCGCCCGAGCCGTTATCCAGCGCCCATCAACTGGCAGAGTTCGTCAGTGGGGAAACGGTACTCGA
TGAATGGTTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCACGAACTTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGCAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTTTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTTCACGATGCGGTTCTACGCTGTTATCGCGTAGCTGAGAATATTGGCGTTCGTGCGATAATGGTTCACGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCGCAAACTTATGAGCGGACTCTGTTCCTTAAACTTCCA
TGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9980.42 Da
• Isoelectric Point: 10.1757

>AT206591 WP_217045451.1 NZ_CP076863:c3351567-3351301 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSPVMEAITQPPAPNDRLKR
AAKRLQTR

Nucleotide

• Length: 267 bp

>AT206591 NZ_CP102492:c59767-59501 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGGGCACTCATCGATGCCGCTGCAGAAATCCTTCA
TAAATCGCGAACCGACTTCATTCTTGAGATGGCCTGCAAGGCTGCGGAGAACGTGATCCTTGATCGTCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCTGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGATGTATAA

Structures

Toxin

• AlphaFold DB: A0A060VJ83