Detailed information of TA system TA206590

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 2740408..2741027
• Replicon: chromosome
• Accession: NZ_CP076863
• Organism: Klebsiella pneumoniae strain V3

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: V1H8E6
• Locus tag: KRP25_RS13445
• Protein ID: WP_001280991.1
• Coordinates: 2740809..2741027 (+)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: KRP25_RS13440
• Protein ID: WP_156649815.1
• Coordinates: 2740408..2740782 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KRP25_RS13430 (2735560)	2735560..2736753	+	1194	WP_217045075.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-
KRP25_RS13435 (2736776)	2736776..2739922	+	3147	WP_217045076.1	multidrug efflux RND transporter permease subunit AcrB	-
KRP25_RS13440 (2740408)	2740408..2740782	+	375	WP_156649815.1	Hha toxicity modulator TomB	Antitoxin
KRP25_RS13445 (2740809)	2740809..2741027	+	219	WP_001280991.1	HHA domain-containing protein	Toxin
KRP25_RS13450 (2741186)	2741186..2741752	+	567	WP_217045077.1	maltose O-acetyltransferase	-
KRP25_RS13455 (2741724)	2741724..2741864	-	141	WP_004147370.1	hypothetical protein	-
KRP25_RS13460 (2741885)	2741885..2742355	+	471	WP_217045078.1	YlaC family protein	-
KRP25_RS13465 (2742330)	2742330..2743781	-	1452	WP_064162152.1	PLP-dependent aminotransferase family protein	-
KRP25_RS13470 (2743882)	2743882..2744580	+	699	WP_217045079.1	GNAT family protein	-
KRP25_RS13475 (2744577)	2744577..2744717	-	141	WP_002892018.1	type B 50S ribosomal protein L36	-
KRP25_RS13480 (2744717)	2744717..2744980	-	264	WP_002892011.1	type B 50S ribosomal protein L31	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-116)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8613.97 Da
• Isoelectric Point: 8.9008

>T206590 WP_001280991.1 NZ_CP076863:2740809-2741027 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Nucleotide

• Length: 219 bp

>T206590 NZ_CP102490:c5246991-5246608 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCATCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14352.02 Da
• Isoelectric Point: 4.8989

>AT206590 WP_156649815.1 NZ_CP076863:2740408-2740782 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANPEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Nucleotide

• Length: 375 bp

>AT206590 NZ_CP102490:c5247233-5246991 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGGATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Toxin

• PDB: 4ICG