Detailed information of TA system TA206579

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mbcAT/RES-Xre
• Location: 7547..8445
• Replicon: plasmid pS2a
• Accession: NZ_CP076859
• Organism: Mycobacterium avium subsp. hominissuis strain OCU901s_S2_2s

Toxin (Protein)

• Gene name: mbcT
• Uniprot ID: A0A859PX34
• Locus tag: BJP78_RS24135
• Protein ID: WP_023870434.1
• Coordinates: 7885..8445 (+)
• Length: 187 a.a.

Antitoxin (Protein)

• Gene name: mbcA
• Uniprot ID: A0A859PYE6
• Locus tag: BJP78_RS24130
• Protein ID: WP_023870435.1
• Coordinates: 7547..7888 (+)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BJP78_RS24100 (BJP78_24955)	2581..3423	+	843	WP_023870443.1	ParB/RepB/Spo0J family partition protein	-
BJP78_RS24105 (BJP78_24960)	3639..3890	+	252	WP_023870442.1	ribbon-helix-helix protein, CopG family	-
BJP78_RS24110 (BJP78_24965)	3887..4303	+	417	WP_023870441.1	type II toxin-antitoxin system VapC family toxin	-
BJP78_RS24115 (BJP78_24970)	4408..4848	-	441	WP_023870440.1	hypothetical protein	-
BJP78_RS24120 (BJP78_24975)	5024..5185	+	162	WP_023870439.1	hypothetical protein	-
BJP78_RS24125 (BJP78_24980)	6307..7458	-	1152	WP_023870436.1	hypothetical protein	-
BJP78_RS24130 (BJP78_24985)	7547..7888	+	342	WP_023870435.1	DUF2384 domain-containing protein	Antitoxin
BJP78_RS24135 (BJP78_24990)	7885..8445	+	561	WP_023870434.1	RES domain-containing protein	Toxin
BJP78_RS24140 (BJP78_24995)	8661..9011	-	351	WP_023870433.1	hypothetical protein	-
BJP78_RS24145 (BJP78_25000)	9008..10711	-	1704	WP_158423352.1	WXG100 family type VII secretion target	-
BJP78_RS24150 (BJP78_25005)	10728..11033	-	306	WP_023870373.1	type VII secretion target	-
BJP78_RS24155 (BJP78_25010)	11109..11414	-	306	WP_134798478.1	hypothetical protein	-
BJP78_RS24160 (BJP78_25015)	11444..12946	-	1503	WP_023870375.1	DUF4226 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	esxN / eccA5 / esxN	1..195724	195724

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (27-148)

Antitoxin

• Antitoxin Xre/MbcA/ParS-like, toxin-binding domain (69-110)

Sequences

Toxin

Protein

• Length: 187 a.a.
• Molecular weight: 20272.58 Da
• Isoelectric Point: 4.8677

>T206579 WP_023870434.1 NZ_CP076859:7885-8445 [Mycobacterium avium subsp. hominissuis]
VSDALDEDLLQRIDARGTTPWSATCFRYTGAHRDPLSGEGARRFGGRWNPPLLFSAIYLSDSAQACMVEVGRAAQAASTT
AEKLLEASYRLHTVEATDLAVLDLTTPEARDTVGLEDDDIHGEDWSACQAVGHAAWFLHMQGILVPAAGGVGLVITAYEQ
RTRPGQLQVSHSEDLTPARYQELRHK

Nucleotide

• Length: 561 bp

>T206579 NZ_CP102489:53443-53748 [Escherichia coli O15:H18]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCCCGCCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGGGAAAATGACATCAAAAATGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12350.16 Da
• Isoelectric Point: 5.0916

>AT206579 WP_023870435.1 NZ_CP076859:7547-7888 [Mycobacterium avium subsp. hominissuis]
MGVNALASTVSSAIERLGLTYEEVGGIVDASARSVARWTSGQVVPQRLNKQRLIELAYVADALAEVLPRDQANVWMFSPN
RLLQHAKPADLVRDGEYQRVLALIDAMAEGIFV

Nucleotide

• Length: 342 bp

>AT206579 NZ_CP102489:53223-53441 [Escherichia coli O15:H18]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGCTGCTCAAGGCGTATGACGTGAATATCTCCGGTCT
GGTCAGTACGACCATGCAGAACGAAGCCCGGCGACTGCGTGCTGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCAG
AGGTCGCCAGGTTTATTGAAATGAATGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Structures

Toxin

• AlphaFold DB: A0A859PX34

Antitoxin

• AlphaFold DB: A0A859PYE6