Detailed information of TA system TA206577

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): sanaTA/AbiEii-DUF6088
• Location: 96488..97836
• Replicon: plasmid pNi1-3
• Accession: NZ_CP076856
• Organism: Shewanella decolorationis strain Ni1-3

Toxin (Protein)

• Gene name: sanaT
• Uniprot ID: A0A5B8R339
• Locus tag: D0436_RS22430
• Protein ID: WP_011711498.1
• Coordinates: 96895..97836 (+)
• Length: 314 a.a.

Antitoxin (Protein)

• Gene name: sanaA
• Uniprot ID: A0A5B8R5A0
• Locus tag: D0436_RS22425
• Protein ID: WP_011711497.1
• Coordinates: 96488..96898 (+)
• Length: 137 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D0436_RS22405 (D0436_24610)	91582..92196	-	615	Protein_89	integrase core domain-containing protein	-
D0436_RS22410 (D0436_24615)	92303..93511	+	1209	WP_001206290.1	IS4-like element IS10A family transposase	-
D0436_RS22415 (D0436_24620)	93525..94474	-	950	Protein_91	IS3-like element ISSpu6 family transposase	-
D0436_RS22420 (D0436_24625)	94525..95445	+	921	WP_254803681.1	hypothetical protein	-
D0436_RS22425 (D0436_24630)	96488..96898	+	411	WP_011711497.1	DUF6088 family protein	Antitoxin
D0436_RS22430 (D0436_24635)	96895..97836	+	942	WP_011711498.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
D0436_RS22435 (D0436_24640)	97844..100486	+	2643	WP_208660715.1	type I restriction-modification system subunit M	-
D0436_RS22440 (D0436_24645)	100488..101726	+	1239	WP_088212909.1	restriction endonuclease subunit S	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..286397	286397

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (37-299)

Antitoxin

• Protein of unknown function DUF6088 (8-130)

Sequences

Toxin

Protein

• Length: 314 a.a.
• Molecular weight: 35771.64 Da
• Isoelectric Point: 7.0965

>T206577 WP_011711498.1 NZ_CP076856:96895-97836 [Shewanella decolorationis]
MKKLKQQFLEVSDALALGNPAIIEKDYWVVALLSELEQLTFETHQMVFSGGTALAKSNVKILRMSEDVDIKLIPTPQFQH
LSRGRKKQYRKALAVKIETAIIASNTFSIEARLVQDEYRYVEYELKYPQQFSQAPCLRPIIKLELMETIPLLALETRGIQ
SLVAELYKLPQEVNGFICVSVNTTLVEKVLSMLRRTMSVKRDAARSDDVTLVRHIYDVHCIHAAQALDNELLAPLFRAIL
AEDIARFGNQHAEFVMDPANELRLGLQEIESNPLFRQRFNDFVKPMVFNSQPPNFDTCFMSFKHIATSLILTI

Nucleotide

• Length: 942 bp

>T206577 NZ_CP102488:4785087-4785494 [Escherichia coli O15:H18]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCGCAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGAAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGTGGCATGATGCTGCGTTTACGTTCTGATAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 137 a.a.
• Molecular weight: 15491.86 Da
• Isoelectric Point: 10.3652

>AT206577 WP_011711497.1 NZ_CP076856:96488-96898 [Shewanella decolorationis]
MAAVDSIFQKIKRSRRYVFERKDFDGIASYDQIGRSLNQLMDRGEIMKLGYGLYTKARVNSLTGRLMPSCPGGSDALMRE
ILKMKDIDFEIDNLSLQSLSGKNTQIPSSVHYSWDQKQFNRKLVVGNRLLTPASRP

Nucleotide

• Length: 411 bp

>AT206577 NZ_CP102488:4784840-4785106 [Escherichia coli O15:H18]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAGTGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCAGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A5B8R339

Antitoxin

• AlphaFold DB: A0A5B8R5A0