Detailed information of TA system TA206575

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pasABC/RelE-HTH
• Location: 70315..70789
• Replicon: plasmid pNi1-3
• Accession: NZ_CP076856
• Organism: Shewanella decolorationis strain Ni1-3

Toxin (Protein)

• Gene name: pasB
• Uniprot ID: A0A8F3ILQ2
• Locus tag: D0436_RS22280
• Protein ID: WP_011839957.1
• Coordinates: 70523..70789 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: A0A8F3IL86
• Locus tag: D0436_RS22275
• Protein ID: WP_011839958.1
• Coordinates: 70315..70539 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D0436_RS22255 (D0436_24460)	67615..67926	-	312	WP_011918375.1	type II toxin-antitoxin system RelE/ParE family toxin	-
D0436_RS22260 (D0436_24465)	67926..68186	-	261	WP_011787805.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
D0436_RS22265 (D0436_24470)	69398..69766	+	369	Protein_61	conjugal transfer transcriptional regulator TraJ	-
D0436_RS22270 (D0436_24475)	69949..70218	+	270	WP_216832063.1	hypothetical protein	-
D0436_RS22275 (D0436_24480)	70315..70539	+	225	WP_011839958.1	DUF6290 family protein	Antitoxin
D0436_RS22280 (D0436_24485)	70523..70789	+	267	WP_011839957.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
D0436_RS22285 (D0436_24490)	70782..71165	+	384	WP_216832064.1	hypothetical protein	-
D0436_RS22290 (D0436_24495)	71188..72186	-	999	WP_254803679.1	replication initiation protein	-
D0436_RS22295 (D0436_24500)	72183..75263	-	3081	WP_011787830.1	Tn3 family transposase	-
D0436_RS22300 (D0436_24505)	75287..75598	-	312	WP_011918375.1	type II toxin-antitoxin system RelE/ParE family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..286397	286397

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-87)

Antitoxin

• Relaxosome protein TraY (4-45)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10355.11 Da
• Isoelectric Point: 9.7697

>T206575 WP_011839957.1 NZ_CP076856:70523-70789 [Shewanella decolorationis]
MAWTIDYSERALKSLKKMDKQNAKRILDFLEQRIAILDDPRTSGKPLKGDLGIFWRYRVGDYRVLCEIQDSKLVILTALI
GHRKEIYE

Nucleotide

• Length: 267 bp

>T206575 NZ_CP102488:4529060-4529356 [Escherichia coli O15:H18]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8303.30 Da
• Isoelectric Point: 4.4079

>AT206575 WP_011839958.1 NZ_CP076856:70315-70539 [Shewanella decolorationis]
MLAIRLPDDIETRLAALADQTGRTKTFYAREAILTHLEDLEDYYLAAQIVSDIRKGAEKTYSSEDVRASLGLDN

Nucleotide

• Length: 225 bp

>AT206575 NZ_CP102488:4529358-4529753 [Escherichia coli O15:H18]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA