Detailed information of TA system TA206573

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 374147..374817
• Replicon: plasmid unnamed1
• Accession: NZ_CP076854
• Organism: Rhizobium sp. WYJ-E13

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: KQ933_RS23310
• Protein ID: WP_216760195.1
• Coordinates: 374398..374817 (+)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: KQ933_RS23305
• Protein ID: WP_216760786.1
• Coordinates: 374147..374401 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KQ933_RS23285 (KQ933_23285)	370080..371132	+	1053	WP_216760191.1	iron ABC transporter permease	-
KQ933_RS23290 (KQ933_23290)	371129..371887	+	759	WP_216760192.1	ABC transporter ATP-binding protein	-
KQ933_RS23295 (KQ933_23295)	371884..373194	+	1311	WP_216760193.1	hypothetical protein	-
KQ933_RS23300 (KQ933_23300)	373187..373696	+	510	WP_216760194.1	hypothetical protein	-
KQ933_RS23305 (KQ933_23305)	374147..374401	+	255	WP_216760786.1	plasmid stabilization protein	Antitoxin
KQ933_RS23310 (KQ933_23310)	374398..374817	+	420	WP_216760195.1	type II toxin-antitoxin system VapC family toxin	Toxin
KQ933_RS23315 (KQ933_23315)	375403..377190	+	1788	WP_216760196.1	PAS domain-containing methyl-accepting chemotaxis protein	-
KQ933_RS23320 (KQ933_23320)	377365..379584	+	2220	WP_216760197.1	EAL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	gmd / htpB / gmd	1..2149667	2149667

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-124)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14944.18 Da
• Isoelectric Point: 4.8668

>T206573 WP_216760195.1 NZ_CP076854:374398-374817 [Rhizobium sp. WYJ-E13]
MILLDTDVISEPWKAAPHEAVVAWLDAQAIEMLFLSAITVAELRFGIAAMPAGKRQTILRDRLEGEVLSHFSERVLPFDL
ASSQFYSDLMARARVSGKAIGAAVGYIAATAASNGLAIATRDTSPFEAAGLEVINPWSR

Nucleotide

• Length: 420 bp

>T206573 NZ_CP102488:c4423771-4423307 [Escherichia coli O15:H18]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCTTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGGCAGAAGAAACCCATTGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9320.53 Da
• Isoelectric Point: 6.4732

>AT206573 WP_216760786.1 NZ_CP076854:374147-374401 [Rhizobium sp. WYJ-E13]
MPAVTVRNLSDETHRALRLRAAHRGRSTEAEIRDILETAVRPPERVKLGSLLAAIGRDAELSDSDVEALQKSRDKTPAEP
MTFE

Nucleotide

• Length: 255 bp

>AT206573 NZ_CP102488:c4424106-4423771 [Escherichia coli O15:H18]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACACC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA