Detailed information of TA system TA206571

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 185859..186433
• Replicon: plasmid unnamed1
• Accession: NZ_CP076854
• Organism: Rhizobium sp. WYJ-E13

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: KQ933_RS22415
• Protein ID: WP_216760050.1
• Coordinates: 186068..186433 (+)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: KQ933_RS22410
• Protein ID: WP_216760768.1
• Coordinates: 185859..186074 (+)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KQ933_RS22385 (KQ933_22385)	181003..182085	+	1083	WP_216760045.1	DUF917 domain-containing protein	-
KQ933_RS22390 (KQ933_22390)	182082..183620	+	1539	WP_216760046.1	hydantoinase/oxoprolinase family protein	-
KQ933_RS22395 (KQ933_22395)	183974..184231	-	258	WP_216760047.1	hypothetical protein	-
KQ933_RS22400 (KQ933_22400)	184551..185159	-	609	WP_216760048.1	J domain-containing protein	-
KQ933_RS33525	185372..185592	+	221	Protein_176	hypothetical protein	-
KQ933_RS22410 (KQ933_22410)	185859..186074	+	216	WP_216760768.1	CopG family transcriptional regulator	Antitoxin
KQ933_RS22415 (KQ933_22415)	186068..186433	+	366	WP_216760050.1	type II toxin-antitoxin system VapC family toxin	Toxin
KQ933_RS22420 (KQ933_22420)	186746..187438	-	693	WP_216760051.1	transporter substrate-binding domain-containing protein	-
KQ933_RS22425 (KQ933_22425)	187450..188214	-	765	WP_216760052.1	4-hydroxy-2-oxoheptanedioate aldolase	-
KQ933_RS22430 (KQ933_22430)	188222..189004	-	783	WP_216760053.1	2-oxo-hepta-3-ene-1,7-dioic acid hydratase	-
KQ933_RS22435 (KQ933_22435)	189025..189918	-	894	WP_216760054.1	intradiol ring-cleavage dioxygenase	-
KQ933_RS22440 (KQ933_22440)	190172..190909	+	738	WP_216760055.1	GntR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	gmd / htpB / gmd	1..2149667	2149667

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-108)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-40)

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 13722.10 Da
• Isoelectric Point: 7.2029

>T206571 WP_216760050.1 NZ_CP076854:186068-186433 [Rhizobium sp. WYJ-E13]
MVKSLFDTNVLIDYLNAVPQARDELTRYREKAISIITWMEVLVGAKPEVAIGTRAFLAGFAVIAVDDAIAERAVSLRQLH
RIKLPDAIIWATANVHSMLLITRNTKDFPREMPDIRMPYEI

Nucleotide

• Length: 366 bp

>T206571 NZ_CP102488:c3817427-3817050 [Escherichia coli O15:H18]
ATGAATACATTACCCGACACTCACGTACGGGAGGCATCGGGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCATCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGGAA
CAATTACAGAATGGTAAATAACATTACCCAGGGCAAGCATCCGGAGGCAAAACGATGA

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 8199.25 Da
• Isoelectric Point: 5.0941

>AT206571 WP_216760768.1 NZ_CP076854:185859-186074 [Rhizobium sp. WYJ-E13]
MRTLVDIGDQEVKALDRLAQREKMSRAALIRKAINDFLARNNADSEAEAFGLWGDRKIDGLTYQENMRSEW

Nucleotide

• Length: 216 bp

>AT206571 NZ_CP102488:c3817851-3817474 [Escherichia coli O15:H18]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCATACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCATCACACTGTATGCGAAGGGGCTGACCTGCGAAGCCGATACCCTTGGCAGTTG
TGGTTACGCTTATCTGGCTGTTTATCCGACACCGGCAGAGCCCGCAATCACCGTATAA