Detailed information of TA system TA206549

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1727516..1728045
• Replicon: chromosome
• Accession: NZ_CP076843
• Organism: Staphylococcus aureus strain 55-100-003

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: KRH49_RS08455
• Protein ID: WP_000621175.1
• Coordinates: 1727516..1727878 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: KRH49_RS08460
• Protein ID: WP_000948331.1
• Coordinates: 1727875..1728045 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KRH49_RS08435 (KRH49_08435)	1724494..1725264	-	771	WP_001041107.1	RNA polymerase sigma factor SigB	-
KRH49_RS08440 (KRH49_08440)	1725239..1725718	-	480	WP_001190825.1	anti-sigma B factor RsbW	-
KRH49_RS08445 (KRH49_08445)	1725720..1726046	-	327	WP_001052491.1	anti-sigma factor antagonist	-
KRH49_RS08450 (KRH49_08450)	1726165..1727166	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
KRH49_RS08455 (KRH49_08455)	1727516..1727878	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
KRH49_RS08460 (KRH49_08460)	1727875..1728045	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
KRH49_RS08465 (KRH49_08465)	1728130..1729278	-	1149	WP_001281139.1	alanine racemase	-
KRH49_RS08470 (KRH49_08470)	1729344..1729703	-	360	WP_000581197.1	holo-ACP synthase	-
KRH49_RS08475 (KRH49_08475)	1729707..1730198	-	492	WP_001286980.1	PH domain-containing protein	-
KRH49_RS08480 (KRH49_08480)	1730191..1731768	-	1578	WP_001294650.1	PH domain-containing protein	-
KRH49_RS08485 (KRH49_08485)	1731761..1732240	-	480	WP_001287081.1	hypothetical protein	-
KRH49_RS08490 (KRH49_08490)	1732449..1733009	-	561	WP_001092419.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T206549 WP_000621175.1 NZ_CP076843:c1727878-1727516 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T206549 NZ_CP102483:2311027-2311392 [Burkholderia contaminans]
ATGGTAAAAGCTCTCTTCGATACCAACATCCTGATCGACTATCTGGGCGGCGTGGAATCCGCTCGTGCGGAACTTGGCCG
CTACGACTATCGGGCGATCAGCACGATCTCGTGGATGGAGGTCCTGGTCGGCACGTCCGCACAGGACGAGGCACCCATCC
GCGCGTGGCTGTCGTCGTTCGATGTCATCGCACTGGACAGCCCGGTTGCGAGTCGTGCCGTGACAATCCGCAAGGAACGC
CGCATACGCCTTCCCGATGCAATCGTCTGGGCATCGGCACAAGTGAACGGTCTACTGCTCGTGTCGCGCAACACGAAGGA
CTTCCCTGCCACCGAACCCGGCGTTCGCGTGCCCTACAAGCTCTGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT206549 WP_000948331.1 NZ_CP076843:c1728045-1727875 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT206549 NZ_CP102483:2310815-2311033 [Burkholderia contaminans]
ATGAGCCGAATTCTGGTCGATCTGTCGGACGGCCAACTCGACGAGTTGGCCGTCATCGTCGAGACTCAACATCGTCCCCG
CGCCGCAATCATCCGCGACGCGATCGATGCCTATATTGCCTTGCACAAGCACCGGCTTGCCGACGACGTCTTCGGTCTCT
GGAAAGATCGCAAGGTCGACGGGCTCACCTATCAGGAAGAACTGCGCTCGGAATGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1